The presence of genes coding for virulence factors was examined by PCR analysis, showing that strain VI possesses the extracellular metalloprotease
A disintegrin-like and metalloprotease
(reprolysin type) with thrombospondin type 1 motifs: the ADAMTS family.
In addition, certain strains also produce a metalloprotease
enterotoxin encoded by the bft gene that enables the bacterium to cause diarrhoea.
Current evidence suggests that a VWF-cleaving enzyme ADAMTS13 (a disintegrin and metalloprotease
with thrombospondin-1-like domain) is deficient or defective in these patients.
It is characterized by a disintegrin and metalloprotease
domain with an epidermal growth factor-like region and harbors both adhesion and proteolytic domains implicated in integrin function and matrix degradation (38, 39).
CD-10, or neprilysin, is a zinc-dependent metalloprotease
that plays a role in degrading the protein Endothelin-1 (ET-1).
regulation of neuropathic pain.
9 is of particular interest because it was demonstrated to be upregulated in activated monocytes and macrophages during FIP (31).
The cysteine proteases (falcipains I, II and III) (10, 11), further degrade the plasmepsins digested products and the generated short peptides finally digested by the metalloprotease
Plasma ADAMTS-13 (A Disintegrin And Metalloprotease
with ThromboSpondin Motifs) activity measured by enzyme-linked immunosorbent assay was reduced at 4% (normal 67-177%) and the working diagnosis of postoperative TTP was established.
The extracellular domain of EPCR is cleaved with a metalloprotease
and the cleavage results in the formation of soluble EPCR (sEPCR) (7).
larvae was further confirmed by two previously described PCR protocols, designed from 16S rRNA gene region (Wiesburg et al, 1991) and 35KDa metalloprotease
precursor gene (Neundorf et al, 2004)