Gill SR, Pop M, Deboy RT, et al Metagenomic
analysis of the human distal gut microbiome.
The process of metagenomic
analysis compared with amplicon-based profiling is summarized in Figure 1.
Techniques such as shotgun metagenomic
sequencing data provides some insight into the metabolic pathways, and as an example, bacterial small sugar transporters were shown to be of particular abundance in the oral cavity sites.
This study will utilize Enterome's state-of-the-art shotgun metagenomic
sequencing technology to profile the human gut microbiome.
Department of Energy Joint Genome Institute (DOE JGI) to generate sequence data from DNA isolated from individual microbes and compared them with metagenomic
(microbial community) information sampled at various depths of Deep Lake.
The Human Microbiome Project and metagenomic
analysis, previously stymied by the requirement for large-scale sequencing of complex microbial communities, was unblocked by applications of 454 and Illumina platforms in 16S rDNA and shotgun sequencing, respectively.
The team at JCVI uses a metagenomic
approach in which the analysis of DNA in an organism is conducted on the entire community of microbes within a sample rather than isolating and culturing each individual microbial species.
The HMP jumpstart phase has made rapid progress not only in reaching its target of 600, 1,000, or 3,000 reference gene sequences but also, to quote the initial announcement, "in continuing 'with metagenomic
analysis to characterize the complexity of microbial communities at individual body sites).
Junjie Qin, "A Human Gut Microbial Gene Caralogue Established by Metagenomic
Sequencing," Nature, no.
Russell Research Center there, used the metagenomic
approach to analyze RNA and DNA viruses associated with two major enteric diseases in poultry: poult enteritis complex and poult enteritis mortality syndrome (see "Metagenomics
Offers Insight Into Poultry Diseases," page 18).
Further study with new methods, such as metagenomic
approaches (Banik and Brady 2010), may lead to discovery of new enzymes for degradation of toxic chemicals from indoor environments.
Their topics include metagenomic
contrasts of viruses in soil and aquatic environments, actinobacterial diversity associated with Antarctic Dry Valley mineral soils, the role of dysbiosis in inflammatory bowel diseases, a targeted metagenomic
approach to determining the "population genome" of marine Synechococcus, the genetic characterization of microbial communities living at the surface of building stones, and novel aromatic degradation pathway genes and their organization as revealed by metagenomic