migration inhibitory factor test

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migration inhibitory factor test

an in vitro test for the production of migration inhibitory factor (MIF) by lymphocytes in response to specific antigens; used for evaluation of cell-mediated immunity. MIF production is absent in certain immunodeficiency disorders, such as wiskott-aldrich syndrome and hodgkin's disease. Called also MIF test.

mi·gra·tion in·hib·i·tor·y fac·tor test

a test that measures the presence of migration inhibitory factor, a 25-kD lymphokine. Usually peritoneal macrophages are placed in a capillary tube in the presence or absence of supernatants from activated T cells in response to immunogenic challenge. If MIF is present, the migration of monocyte/macrophages is reduced.

migration inhibition assay

An immune function test that measures MIF (monocyte/macrophage inhibitory factor) and LIF (leukocyte inhibitory factor) production after lymphocyte stimulation with common antigens—e.g., streptokinase-streptodornase, Candida antigen, PPD, concanavalin A, phytohemagglutinin, pokeweed mitogen, etc.
In the MIA, peripheral lymphocytes are harvested from peripheral blood, spleen or lymph nodes, washed, and then separated from the plasma by centifugation; the cells are then exposed to the mitogen, washed again, and then incubated. The supernatent is then used to determine the inhibition of migration on a sample of naïve macrophages (e.g., from guinea pigs). The MIA correlates well with immune competence, and is one of the best tests for delayed-type hypersensitivity. MIF production is lost or markedly decreased in immunodeficiency states such as AIDS, DiGeorge syndrome and Wiskott-Aldrich syndrome.
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