The amplified products for H-FABP and MYOG genes were digested with MspI restriction endonuclease, separated on a 2.
k] represents the GHRH (k = AA, AB, BB), H-FABP (k = DD, DR, RR), and MYOG (k = AA, AB, BB) genotypes, and [e.
In the case of the MYOG gene, when the 353 bp PCR products were digested with MspI restriction enzyme, AA (353 bp), BB (219 and 134 bp) and AB types (353, 219, and 134 bp) appeared (Figure 1C).
Examination of the association between breed and the MYOG gene, showed higher frequency of BB genotype for all of the three breeds, Yorkshire, Landrace, and Duroc, (56.
The polymorphisms of the GHRH, H-FABP, and MYOG genes employed in this study were highly associated with breed.
For the MYOG gene, there was no significant effect of MspI polymorphic sites on the economic traits in this study.