Hepatoid carcinomas may also express MOC31
and mCEA, which are infrequently expressed in HCCs.
Histologic specimens obtained at surgery were fixed in 10% formalin, routinely processed and embedded in paraffin All specimens were evaluated immunohistochemically with antibodies calretinin, vimentin, CK5/6, MOC31
, CD15 and CEA using staining procedures.
The diagnostic utility of MOC31
, BerEP4, RCC marker and CD10 in the classification of renal cell carcinoma and renal oncocytoma: An immunohistochemical analysis of 328 cases.
17) In contrast, CA9, (13,19) MOC31
, (11) c-kit, (19) VEGFR-2, (19) PDGFR-[alpha], (19) and TFE (18) were not detected.
The most useful general carcinoma markers are MOC31
, BG8, carcinoembryonic antigen, and BerEp4.
Lung Adenocarcinoma Versus Mesothelioma (Second IHC Panel) Marker Lung Adenocarcinoma Mesothelioma Napsin A + - MOC31
+ - CEA + - CK5/6 - + Thrombomodulin -/+ + HBME1 -/+ + Abbreviations: CEA, carcinoembryonic antigen; CK5/6, cytokeratin 5/ 6; HBME1, Hector Battifora mesothelial/1; IHC, immunohistochemical; +, greater than 75% of cases are positive; -, fewer than 5% of cases are positive; -/+, fewer than 50% of cases are positive.
Among them, MOC31
has been demonstrated to be one of the best epithelial markers expressed in a broad spectrum of carcinomas.
The tumor cells were negative for pancytokeratin, cytokeratin 7, cytokeratin 20, mammaglobin, MOC31
, PAX-8, estrogen receptor, TTF1, polyclonal carcinoembryonic antigen, CDX2, BerEP4, Hep Par 1, calretinin, synaptophysin, CD56, CD10, S100, Melan-A, desmin, actin, CD34, factor 8, and inhibin.
Alternative epithelial markers in sarcomatoid carcinomas of the head and neck, lung, and bladder--p63, MOC31
, and TTF-1.
Original Diagnosis Consult Type Diagnosis Cervical biopsy 3 SIL Benign Prostate biopsy 4 Carcinoma Benign Breast biopsy 3 Invasive carcinoma DCIS arising in DCIS Pleural fluid 1 Adenocarcinoma Benign Stomach biopsy 1 Adenocarcinoma Benign granulation tissue Biopsy Site/ Comment Method to Potentially Prevent Error Type Cervical biopsy No IHC done p16, MIB-1 in selected cases Prostate biopsy No IHC done p63, HMWK, AMACR in selected cases Breast biopsy No IHC done p63 in selected cases Pleural fluid No IHC done BER-EP4, MOC31
in selected cases Stomach biopsy No IHC done Keratin, CD68 in selected cases Abbreviations: DCIS, ductal carcinoma in situ; HMWK; high-molecular-weight keratin; IHC, immunohistochemistry; SIL, squamous intraepithelial lesion.
, BG8, and BerEP4 all have high sensitivity and specificity.
2, CK [cytokeratin] 7, CK20, CK17, CK19, MUC1, MUC2, MUC4, MUC5AC, MUC6, p53, DPC4/ SMAD4, CDX2, pVHL [von Hippel-Lindau tumor suppressor gene protein], S100P, IMP-3 [insulin-like growth factor 2 messenger RNA binding protein 3], maspin, mesothelin, claudin 4, claudin 18, annexin A8, fascin, PSCA [prostate stem cell antigen], MOC31
, CEA [carcinoembryonic antigen], and CA19-9 [cancer antigen 19-9]) in the diagnosis of ductal adenocarcinoma of the pancreas by using 60 cases of pancreatic DAC on routine and tissue microarray (TMA) sections.