MIB1

MIB1

A monoclonal antibody which recognises a formalin-fixation-resistant epitope on the cell proliferation-associated Ki-67 antigen. MIB1 is a reliable marker of cell proliferation, and can be used for image analysis of paraffin-embedded tissues; Ki-67 is a short-lived nonhistone protein expressed during the G1, S and G2/M phases of the cell cycle, but not in the G0 (resting period) phase.

Image analysis of MIB1 may be an alternative method for objectively, reproducibly and reliably classifying dysplastic changes of the cervical epithelium. Its presence is regarded as an independent prognostic indicator in breast cancer—the presence of MIB-1 is associated with a slight increased tumour aggressiveness, but is a poor indicator of prognosis.
References in periodicals archive ?
Mucin stains and immunohistochemistry were performed subsequently using the following antibodies: S100, cytokeratin, CD34, CD68, synaptophysin, chromogranin, CD56, smooth muscle actin, CD117, HMB45, and MIB1 (Table 1).
A unique membranous and cytoplasmic reaction with Ki-67 (Dako MIB1 antibody) is different from the usual nuclear reaction with this antibody (figure 2).
Cyclin D1, p53, MIB1, intratumoral microvessel density, and human papillomavirus in advanced laryngeal carcinoma: association with nodal metastasis.
Ki 67 immunohistochemistry : Ki 67 immunohistochemistry was performed with the clone MIB1 (Immunotech, Int, Marseilles, France) using a highly sensitive streptavidin-biotin-peroxidase technique with a commercial kit (BIO SPA, Milan, Italy).
Estrogen receptor staining (2-3+) was present in 70% of tumor nuclei; progesterone receptor (3+), 75%; and MIB1, 20% and focally 40% to 50%.
Immunohistochemical stains in the differential diagnosis with nested variant of urothelial carcinoma, including MIB1, CK20, p27, and p53, are not useful and the diagnosis relies on routine histology.
Molecular grading of urothelial cell carcinoma with fibroblast growth factor receptor 3 and MIB1 is superior to pathologic grade for the prediction of clinical outcome.
Some markers, such as antibodies against p53, MIB1 (Ki-67), cyclin D1, [beta]-catenin, and racemase, may be helpful in difficult cases, but all have limitations.
MIB1 is a proliferation marker expressed in proliferating cells.
Paraffin section storage and immunohistochemistry: effects of time, temperature, fixation, and retrieval protocol with emphasis on p53 protein and MIB1 antigen.
24,25) To facilitate the identification of proliferating melanocytes, we have recently developed a homegrown cocktail that includes anti-MART-1 and MIB1 (against Ki-67).
Ki-67 immunostains with MIB1 indicate high proliferative activity ([greater than or equal to] 10%) in 72% of cases (Figure 4, A through D).