MIB1

MIB1

A monoclonal antibody which recognises a formalin-fixation-resistant epitope on the cell proliferation-associated Ki-67 antigen. MIB1 is a reliable marker of cell proliferation, and can be used for image analysis of paraffin-embedded tissues; Ki-67 is a short-lived nonhistone protein expressed during the G1, S and G2/M phases of the cell cycle, but not in the G0 (resting period) phase.

Image analysis of MIB1 may be an alternative method for objectively, reproducibly and reliably classifying dysplastic changes of the cervical epithelium. Its presence is regarded as an independent prognostic indicator in breast cancer—the presence of MIB-1 is associated with a slight increased tumour aggressiveness, but is a poor indicator of prognosis.
References in periodicals archive ?
Prognostic significance of the S-phase and MIB1 (Ki-67) proliferation parameters in node-negative breast carcinoma [in German].
The prognosis depends upon tumor type, grade, stage and MIB1 proliferation index.
Assessment of the new proliferation marker MIB1 in breast carcinoma using image analysis: associations with other prognostic factors and survival.
Effect of mitomycin-C on human foreskin fibroblasts used as feeders in human embryonic stem cells: Immunocytochemistry MIB1 score and DNA ploidy and apoptosis evaluated by flow cytometry.
Antibody Clone Source Dilution Control 1 MSA HHF35 S1 1:1000 Myometrium 2 SMA 1A4 S1 1:1500 Myometrium 3 Calponin CALP S1 1:200 Myometrium 4 Desmin D33 S1 1:1000 Myometrium 5 Myogenin F5D S1 1:100 Rhabdomyosarcoma 7 ALK1 ALK1 S1 1:300 Anaplastic lymphoma 8 AE1/AE3 AE1/AE3 S1 1:100 Skin: epidermis 9 CD34 QBEND10 S1 1:25 Skin: Blood vessels 10 S100 polyclonal S1 1:1000 Skin: Nerves 11 CD117 C--KIT S1 1:500 Gastric stromal tumour 12 HHV8 13B10 S2 1:40 Kaposi's sarcoma 13 p53 DO7 S1 1:100 Nodal lymphocytes 14 Ki67 MIB1 S1 1:40 Nodal lymphocytes Result No.
A unique membranous and cytoplasmic reaction with Ki-67 (Dako MIB1 antibody) is different from the usual nuclear reaction with this antibody (figure 2).
Cyclin D1, p53, MIB1, intratumoral microvessel density, and human papillomavirus in advanced laryngeal carcinoma: association with nodal metastasis.
Ki 67 immunohistochemistry : Ki 67 immunohistochemistry was performed with the clone MIB1 (Immunotech, Int, Marseilles, France) using a highly sensitive streptavidin-biotin-peroxidase technique with a commercial kit (BIO SPA, Milan, Italy).
F, MIB1 proliferative index is higher than 95% (Diff-Quik, original magnification X600 [A]; Papanicolaou stain, original magnification X600 [B]; hematoxylin-eosin, original magnification X600 [C]; immunohistochemistry, original magnifications X400 [D and E] and X600 [F]).
Immunohistochemical staining with MIB1, bcl2 and p16 assists in the distinction of cervical glandular intraepithelial neoplasia from tubo-endometrial metaplasia, endometriosis and microglandular hyperplasia.
Uterine leiomyosarcomas are characterized by high p16, p53 and MIB1 expression in comparison with usual leiomyomas, leiomyoma variants and smooth muscle tumours of uncertain malignant potential.