For example, in vitro experiments have demonstrated that MEHP induces adipogenesis by activating PPAR-y (Feige et al.
i) DEHP metabolites included MEHP, MEHHP, MEOHP, and MECPP; butyl phthalate metabolites, MBP and MiBP; total phthalate metabolites, MEP, MEHP, MEHHP, MECPP, MEOHP, MBzP, MBP, and MiBP.
To conduct the study, Hixon and graduate student Benjamin Moyer fed either pure corn oil or corn oil laced with three different concentrations of MEHP (100, 500 and 1,000 milligrams per kilogram of body weight) to pregnant mice in days 17 to 19 of their term.
The story the evidence from the paper seems to tell is that a high level of in utero exposure to MEHP causes female mice to have a shortened but more intense reproductive life, possibly because greater hormone levels induce an elevated number of follicles to release more eggs per estrus cycle.
Pubertal jinekomasti ve kontrol gruplari ile her iki grubu kapsayan tum calisma grubunda plazma DEHP, plazma MEHP ve pankreatik [alpha]-amilaz ve lipaz duzeyleri arasindaki iliskiye ait Pearson korelasyon katsayilari Tablo 2'de verilmistir.
Pubertal jinekomasti tanisi almis cocuklarda tarafimizdan yapilan calismada gerek DEHP gerekse ana metaboliti MEHP duzeylerinin kontrol grubuna oranla istatistiksel olarak anlamli olcude yuksek oldugu belirlenmis ve pubertal jinekomastinin patojenezinde fitalatlarin rolu olabilecegi konusunda onemli bir bulgu saglanmistir (14).
The researchers didn't find statistical evidence of a relationship between MEHP
and low birth weight or any other deleterious outcome, however.
MEHP concentrations (Table 3) and other DEHP metabolites (see Supplemental Material, Table S2) were higher in African-American compared with white mothers but no other differences were observed.
MEHP was inversely associated with the highest BMI category, and MBzP was positively associated with the highest BMI category, but otherwise associations with urinary phthalate metabolites were close to the null (data not shown).
5) ([micro]g/L) Abbreviations: BE, biomonitoring equivalent; DEHP, di(2-ethylhexyl)phthalate; [SIGMA](GM), sum of geometric means of MEHP
, and 5oxo-MEHP
; HBM-I, human biomonitoring value I; HBM-II, human biomonitoring value II; JECFA, Joint FAO/WHO Expert Committee on Food Additives; LOQ, limit of quantification; MBzP, monobenzyl phthalate; MEP, monoethyl phthalate; MiBP, monoisobutyl phthalate; MnBP, mono-n-butyl phthalate; P90, 90th percentile.
Rosiglitazone and MEHP were used as positive controls.
To investigate the elution profile of chemicals in the gel permeation chromatography (see Supplemental Material, "Operation of Gel Permeation Chromatography"), DEHP, MEHP, TBBPA, TBBA, and other tested compounds were quantitatively analyzed by either liquid chromatography (LC) tandem mass spectrometry (LCMS) (Agilent 6410 Triple Quad LCMS; Agilent Technologies, Santa Clara, CA), or gas chromatography coupled with mass spectrometry detector (GC-MSD).