However, few studies evaluate the relationship of miR-143 and MAP3K7 in BC, and the mechanism and function of miR-143 in the occurrence and development of BC are still not fully understood.
In the present study, we compared the levels of miR-143, ERK5, and MAP3K7 in BC tissues, noncancerous tissues and normal breast tissues.
To investigate the relationship of miR-143 and ERK5, MAP3K7 or cyclin D1, cells were co-transfected with miR-143 mimic/inhibitor and small interfering RNA targeting (si)-ERK5, si-MAP3K7 or si-cyclin D1 (100 nm, Qiagen).
Then, the levels of miR-143, ERK5 and MAP3K7 were detected using SYBR[R] Premix Ex Taq[TM] (TaKaRa, Japan).
Next, sections were blocked with 1% serum, and then incubated with rabbit anti-human ERK5, phospho-ERK5 (p-ERK5), MAP3K7 or phospho-MAP3K7 (p-MAP3K7) polyclonal antibody (1:50, Santa Cruz, USA) at 4[degrees]C overnight.
The membranes were probed with mouse anti-phospho-ERK5 (p-ERK5), ERK5, p-MAP3K7, MAP3K7 and cyclin D1 polyclonal antibodies (1:500, Santa Cruz) and mouse anti-GAPDH monoclonal antibody (1:2000, Sigma) overnight at 4[degrees]C, respectively.
Pearson correlation analysis was used to evaluate the correlation of miR-143 with ERK5 or MAP3K7.
Relationship of miR-143 and ERK5 or MAP3K7 in BC tissues
MAP3K7 is linked to IL1 receptor and tumor necrosis factor (TNF) receptor signalings (Sato et al.
Dominant-negative MAP3K7 induces GO exit in the quiescent liver and accelerates hepatic cell cycle progression during regeneration (Bradham et al.
Gene name Gene description MAP3K7 Mitogen-activated protein kinase kinase kinase 7 HDAC9 Histone deacetylase 9 CFTR Cystic fibrosis transmembrane conductance regulator ETHE1 Ethylmalonic encephalopathy 1 MCM3AP Minichromosome maintenance deficient 3 associated protein SPIB Spi-B transcription factor WT1 Wilms' tumor 1 BID BH3 interacting domain death agonist C1QTNF3 Clq and tumor necrosis factor related protein 3 S0D2 Superoxide dismutase 2 SFRS1 Splicing factor, arginine/serine-rich 1 UBE2N Ubiquitin-conjugating enzyme E2N Gene name Glycyrrhizin Silymarin Change (folds) FDR Change (folds) FDR MAP3K7 -5.
B binding protein Transforming growth factor, beta 2 TCFBR2 Transforming growth factor, beta receptor II TCF/Smad pathway SMAD1 SMAD family member 1 SMAD6 SMAD family member 6 S0X4 SRY (sex determining region Y)-box 4 SP1 Spl transcription factor CTNNB1 CREB binding protein Ras/MAPK(non-Smad) RHOA Ras homolog gene family, pathway member A RH08 Ras homolog gene family, member B MAP3K7
Mitogen-activated protein kinase kinase kinase 7 MAPK14 Mitogen-activated protein kinase 14 Downstream effector BCL2L1 BCL2-like 1 CDC6 Cell division cycle 6 homolog CDKN1B Cyclin-dependent kinase inhibitor 1B CEBPB CCAAT/enhancer binding protein (C/EBP), beta FN1 Fibronectin 1 CADD45B Growth arrest and DNA-damage-inducible.