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luciferase

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luciferase
(loosif´rās´),
n an enzyme present in certain luminous organisms that act to bring about the oxidation of luciferins; energy produced in the process is liberated as bioluminescence.


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The cells were then treated for 24 hr and harvested and assayed for luciferase and [beta]-galactosidase activities using the Luciferase Assay System (Promega Corporation, Madison, WI, USA) and the [beta]-Galactosidase Enzyme Assay System (Promega).
The kit includes a genetically engineered cell line that produces the reporter enzyme luciferase in response to specific TSI binding, and the amount of luciferase produced is then rapidly detected using a luminometer.
The system is ideal for measuring flash and glow assays, including dual luciferase and acridinium ester flash assays, in both 96-well and 384-well plates.
 
 
 
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