Locke's solution was used for extracellular recording, of which composition was: 140 mM NaCl, 5.
The nerve was immediately placed in a Petri dish containing ice cold (4[degrees]C) modified Locke's solution and they were used for experimental recording in the same day.
The preparations were placed in isolated organ baths, incubated in Locke's solution at 37 [+ or -] 1[degrees]C and bubbled with gas (95% [O.
A uterine horn was incubated in Locke's solution and equilibrated for 45 min.
As is well known, Locke's solution in the Letter opposes the state enforcing religious practice.
Ironically, Locke's solution recreates one of the key problems of state-controlled religion: the nature of the church's relation to the state prevents one from addressing the other.
2] inhalation, DRG were dissected and immediately placed in an ice-cold modified Locke's solution.
The composition of modified Locke's solution was: 140 mM NaCl, 5.
Stock solutions were added to the modified Locke's solution for intracellular recording and to modified external solutions for patch-clamp recording so as to provide a desired concentration of estragole with a final concentration of DMSO always lower than 0.
Pharmacological agents were dissolved in Locke's solution and administered via the bath.
Modified Locke's solution (composition in mM: 140 NaCl, 5.
Each testing extract was dissolved in Locke's solution
(in mM: 154 NaCl, 5.