Lobeline, a piperidine alkaloid, which is produced by Lobelia inflata (family Lobeliaceae) and several other Lobelia species, stimulates chemoreceptors in carotid and aortic bodies, and then exerts reflectory activation of respiratory centre (Fig.
We could show for the first time in our studies that lobeline can moderately inhibit P-gp mediated efflux and reverse P-gp dependent resistance at non-toxic concentrations.
Lobeline, verapamil, doxorubicin, MTT, rhodamine 123, G418, mitoxantrone, and other chemicals were from Sigma Aldrich.
Lobeline was applied at different concentrations into wells 20min after 1[micro]g/ml rhodamine 123 had been added.
Cells were washed and incubated with 20[micro]M lobeline at 37 [degrees]C for 120 min.
Different concentration of lobeline or different concentration of doxorubicin and 10[micro]M lobeline were added into three triplicate wells after cells were incubated for 24 h at 37 [degrees]C and 5% [CO.
Inhibition efficiency was defined as (fluorescence intensity of lobeline treated cells minus fluorescence of untreated control)/(fluorescence intensity of verapamil treated minus fluorescence of untreated control).
2, the rhodamine fluorescence of Caco-2 cells is positively correlated with an increase of lobeline concentration.
However, in the lobeline treated group the signals shifted to lower right region indicating that cells now retained rhodamine 123 (Fig.
In order to test if P-gp mediated MDR can be overcome by lobeline a reversal assay was conducted.
This approval will complement DynaGen's issued patent's for sublingual administration of lobeline, granted May 9, 1995 (U.
Our goal of establishing broad patent protection for the use of lobeline and lobeline analogues as aids to smoking cessation has essentially been accomplished, particularly for the United States," said Indu Muni, Ph.