Possible targets for barrier-influencing moisturizing creams include the intercellular lipid bilayers
, where the fraction of lipids forming a fluid phase might be changed due to compositional or organizational changes.
Software on the chip controls the insertion of the nanopores into the lipid bilayers
and allows for active control of individual sensors on the array.
They cover amperometric biosensors, imaging of single biomolecules by scanning tunneling microscopy, applications in reflectivity in bioelectrochemistry, model lipid bilayers
at electrode surfaces, enzymatic fuel cells, Raman spectroscopy of biomolecules at electrode surfaces, membrane electroporation in high electric fields, and electroporation for medical use in drug and gene electrotransfer.
The ultrastructure of C5b-8 has no pore-like features; however, this complex causes leakage of synthetic lipid vesicles, increases ion-conductance in planar lipid bilayers
, and promotes the slow osmotic lysis of simple cells such as heterologous erythrocytes.
Despite its proven value, bilayer recording can be very frustrating due to the capricious nature of lipid bilayers
which have to be formed manually one by one and which often lack stability.
Surface energies of supported lipid bilayers
, optionally covered by PEG, were quantified with contact angle goniometry and AFM.
The influences on both erythrocytes and planar lipid bilayers
are formed at the top of the wells and nanopore constructs (a processive exonuclease enzyme combined with an a-hemolysin nanopore containing an adapter molecule, cyclodextrin) are added to the chip to sit within the lipid bilayers
, forming a single hole in the bilayer.
The technique has the potential to model whole cells and tissues with sufficient resolution to differentiate lipid bilayers
Among the topics in this edition are using lipidic advances in imaging secondary ion mass spectrometry for biological samples, electron crystallography as a technique to study the structure of membrane proteins in a lipidic environment, insights from type II diabetes into the interplay of catalysis and toxicity by amyloid intermediates in lipid bilayers
, biochemical and structural properties of the integrin-associated cytoskeleton protein talin, bioimage informatics for experimental biology, and determining the structure and dynamics of membrane proteins by magic angle spinning solid-state nuclear magnetic radiation.
It is fitting that the discovery of a new protein capable of fusing membranes comes 10 years after the demonstration that SNAREs can fuse lipid bilayers
," said Daga, a researcher at the Eugenio Medea Scientific Institute in Conegliano, Italy.
Outstanding experiments presented in  showed that the CoF between two hydrogel surfaces imitating cartilage in the presence of lipid bilayers
separated by a physiological salt solution (Fig.