Elastic modeling of biomembranes and lipid bilayers
We presented two conformations of a bacterial Nav from Caldalkalibacillus thermarum reconstituted into lipid bilayers
in a single crystal at 9[Angstrom] resolution based on electron crystallography.
In stochastic sensing, a single protein nanopore is placed in a lipid bilayer
, and the ionic current driven through it by an applied potential is monitored.
They cover amperometric biosensors, imaging of single biomolecules by scanning tunneling microscopy, applications in reflectivity in bioelectrochemistry, model lipid bilayers
at electrode surfaces, enzymatic fuel cells, Raman spectroscopy of biomolecules at electrode surfaces, membrane electroporation in high electric fields, and electroporation for medical use in drug and gene electrotransfer.
Despite its proven value, bilayer recording can be very frustrating due to the capricious nature of lipid bilayers
which have to be formed manually one by one and which often lack stability.
The presence of a surfactant increases the elasticity of the lipid bilayers
Surface energies of supported lipid bilayers
, optionally covered by PEG, were quantified with contact angle goniometry and AFM.
The influences on both erythrocytes and planar lipid bilayers
The technique has the potential to model whole cells and tissues with sufficient resolution to differentiate lipid bilayers
Among the topics in this edition are using lipidic advances in imaging secondary ion mass spectrometry for biological samples, electron crystallography as a technique to study the structure of membrane proteins in a lipidic environment, insights from type II diabetes into the interplay of catalysis and toxicity by amyloid intermediates in lipid bilayers
, biochemical and structural properties of the integrin-associated cytoskeleton protein talin, bioimage informatics for experimental biology, and determining the structure and dynamics of membrane proteins by magic angle spinning solid-state nuclear magnetic radiation.
It is fitting that the discovery of a new protein capable of fusing membranes comes 10 years after the demonstration that SNAREs can fuse lipid bilayers
," said Daga, a researcher at the Eugenio Medea Scientific Institute in Conegliano, Italy.
In their absence, water only trickles across the hydrophobic lipid bilayers
of cell membranes.