5, 5 and 10 [micro]M for cryptotanshinone, and 0, 5, 10 and 20 [micro]M for salvianolic C) were used for the construction of Lineweaver-Burk plot
and estimation of [K.
max] were then calculated using the Lineweaver-Burk plot
4 mg/ml starch, respectively, as determined by Lineweaver-Burk plot
m]s) were estimated from six experiments using the Lineweaver-Burk plot
The data were fitted to Michaelis-Menten model and further analyzed using Lineweaver-Burk plot
and Dixon plot.
This was indicated by the comparable Michaelis constant (Km) values obtained from the Lineweaver-Burk plot
As for eupatorin, Lineweaver-Burk plot
illustrated in Fig.
max]) were derived from Lineweaver-Burk plot
while inhibition constant ([K.
These plots included Primary Lineweaver-Burk Plot
(obtained by reciprocal of reaction velocities versus reciprocal of substrate concentrations), Dixon plot (obtained by reciprocal of reaction velocities versus inhibitor concentrations), Secondary Lineweaver-Burk plot
Ki value was calculated by Secondary Lineweaver-Burk plot
using the slopes of the lines in the Primary Lineweaver-Burk plot
versus inhibitor concentrations, while the Secondary Lineweaver-Burk plot
of y-intercept in primary plot versus inhibitor concentrations was used to obtain [alpha][K.
A Lineweaver-Burk Plot
is a double reciprocal plot in which varying substrate concentrations are plotted against reaction velocities to obtain linear transformation.
Based on analysis of nonlinear regression of inhibition data and primary Lineweaver-Burk plot
and secondary plot presented in pooled human liver microsomes (Fig.