Lambda phage

(redirected from Lambda DNA)

Lamb·da phage

a bacteriophage used extensively in experimental systems.
References in periodicals archive ?
Extracted DNA was further purified using the phenol-chloroform method and quantified by analyzing DNA on 1% agarose gel using diluted uncut lambda DNA as standard.
Aliquots (1 [micro]L) of purified PCR products were quantified by comparison with serial dilutions of uncut lambda DNA (Promega) in 1% agarose gels (Fig.
1U corresponds to the amount of enzyme required to digest 1 [micro]g of lambda DNA in 1 h at 37[degrees]C in 50 [micro]l of assay buffer.
Restriction digestion of Lambda DNA and DNA from African individuals: To check for the effects of PCR components on the activity of enzymes, the digestion pattern of pure Lambda DNA (taken as positive control of digestion) was analyzed with respect to Lambda DNA spiked with PCR amplicon in three different forms: (a) untreated PCR amplicon spiked with Lambda DNA; (b) salt precipitated PCR amplicon spiked with Lambda DNA; and (c) column precipitated PCR amplicon spiked with Lambda DNA.
If more detailed studies document that lambda DNA indeed has a short half-life in human specimen matrices, then its range of applications will be limited.
Quantifying was done using agarose minigel electrophoresis with lambda DNA as the standard.
coli that was exposed to lead showed very few fragments compared to Lambda DNA and E.
The difference between states is not due to variation in DNA sequence, but rather variation in which regulatory proteins are bound to the control regions of the lambda DNA.
The quantity of DNA was also confirmed by comparing with Quantification Standards Phage Lambda DNA (Fermentas) on 0.