A gene on chromosome 2p11 that encodes a hepatic fatty acid-binding protein that binds long-chain fatty acids, other hydrophobic ligands and bile acids; it may be involved in intracellular lipid transport.
In the present study, L-FABP mRNA expression was increased by CORT-induced stress, which may enhance the absorption of non-esterified fatty acid by intestinal epithelium from digesta.
CORT treatment caused an increased expression of SGLT1, CaBP-D28k, PepT1 mRNA in the duodenum and L-FABP mRNA in the jejunum as a compensation for the loss of absorptive area of the small intestine.
The primer sequence of different nutrient transporters and the house-keeping gene [beta]-actin for quantitative RT-PCR Name Oligo Primer sequence SGLT1 Forward primer 5'-GATGTGCGGATACCTGAAGC-3' Reverse primer 5'-AGGGATGCCAACATGACTGA-3' PepT1 Forward primer 5'-TACGCATACTGTCACCATCA-3' Reverse primer 5'-ATGGATGGGAAGGAGCTACAA-3' CaBP-D28k Forward primer 5'-ATGGATGGGAAGGAGCTACAA-3' Reverse primer 5'-TGGCACCTAAAGAACAACAGGAAAT-3' L-FABP Forward primer 5'-GAAGGGTAAGGACATCAA-3' Reverse primer 5'-TCGGTCACGGATTTCAGC-3' [beta]-actin Forward primer 5'-CCACCGCAAATGCTTCTAAAC-3' Reverse primer 5'-AAGACTGCTGCTGACACCTTC-3' Name Predicted size (bp) Genebank accession SGLT1 225 AJ236903 PepT1 205 AY029615 CaBP-D28k 194 M14230 L-FABP 219 NM_204192 [beta]-actin 175 NM_205518 Table 2.
To determine the biological variation of circulating L-FABP on the reference interval, plasma samples were obtained, as described previously (7,8), from 80 healthy individuals (40 males and 40 females) to study the influence of age and gender and from another 12 healthy individuals (6 males and 6 females) to study the influence of circadian rhythm.
A paired Wilcoxon signed-rank test was used to assess any difference between the increases in serum L-FABP, ALT, and [alpha]-GST.
Healthy individuals had a median plasma L-FABP concentration of 9.