Cloning (redirected from Klone)

clon·ing (klnng)

n.

The transplantation of a nucleus from a somatic cell into an ovum, which then develops into an embryo.

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cloning

[klō′ning]

a procedure for producing multiple copies of genetically identical organisms or cells or of individual genes. Organisms may be cloned by transplanting blastocysts from one embryo into an empty zona pellucida, or nuclei from the cells of one individual into enucleated oocytes. Cells may be cloned by growing them in culture under conditions that promote cell reproduction. Genes may be cloned by isolating them from the genome of one organism and incorporating them into the genome of an asexually reproducing organism, such as a bacterium or a yeast.

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Cloning

The generation of an exact living replica of an organism’s DNA—DNA cloning—or a cell—cell cloning—produced asexually from a single ancestor
Biotechnology DNA cloning in recombinant technology, DNA manipulation to produce multiple copies of a single gene or DNA segment
Genetics The process of asexually producing a group of genetically identical cells or clones, all from a single ancestor
Molecular biology The production of multiple, genetically identical molecules of DNA, cells, or organisms, which involves reverse transcription of purified mRNA into the corresponding cDNA before insertion into a vector, the synthesis of multiple copies of a DNA sequence, previously introduced into E coli, grown in the bacteria in culture media, removed, and DNA segments of interest isolated

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cloning

see recombinant DNA technology.

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directional cloning

the insertion of a segment of foreign DNA which has a defined polarity, e.g. different restriction enzyme sites at each end, into a plasmid vector.