Low concentrations of KLK4 were found in a wide variety of tissues, adult and fetal.
KLK4 was primarily found in seminal plasma, with lower concentrations in breast milk and urine.
6] Human genes: KLKI, kallikrein 1, renal/pancreas/salivary; KLK2, kallikrein 2, prostatic; KLK3, kallikrein 3 (prostate-specific antigen); and KLK4
, kallikrein 4 (prostase, enamel matrix, prostate).
All of the above studies have examined total KLK4 mRNA expression.
In contrast to the multiple studies examining KLK4 at the mRNA level, only 3 published studies have investigated the KLK4 gene product (the hK4 protein).
AJ310938) and KLK4 variant 1 for the KLK4 gene (GenBank accession no.
AJ310938) and KLK4 variant 1 (AF148532), we designed primers that bound in coding exons 3 and 5 (F1 and R1 primers, respectively; Fig.
KLK7 is up-regulated in ovarian cancer (62), and KLK4 and KLK5 are indicators of poor prognosis of ovarian cancer (63-65).
An alternatively spliced variant of KLK4 expressed in prostatic tissue.
Because the open reading frame of KLK4 predicts that its first 26 N-terminal amino acids are the signal peptide, we expected that the 224-amino acid mature enzymatic form would be a secreted protein (3, 5, 9).
After the PCRs were completed, 15 [micro]L of the 278-bp KLK4 product was visualized by staining with ethidium bromide on 2% agarose gels.
New gene Previous gene New protein symbol (a,b) symbol(s) symbol KLK1 KLK1 hK1 KLK3 KLK3 hK3 KLK2 KLK2 hK2 KLK4
PRSS17, KLK-L1, KLK4
hK4 KLK5 KLK-L2 hK5 KLK6 PRSS9 hK6 KLK7 PRSS6 hK7 KLKS PRSS19 hK8 KLK9 KLK-L3 hK9 KLK10 PRSSL1, hK10 KLK11 PRSS20 hK11 KLK12 KLK-L5 hK12 KLK13 KLK-L4 hK13 KLK14 KLK-L6 hK14 New gene symbol (a,b) Other protein names/symbols KLK1 Pancreatic/renal kallikrein, hPRK KLK3 Prostate-specific antigen, PSA KLK2 Human glandular kallikrein 1, hGK-1 KLK4
Prostase, KLK-L1 protein, EMSP1 KLK5 KLK-L2 protein, HSCTE KLK6 Zyme, protease M, neurosin KLK7 HSCCE KLKS Neuropsin, ovasin, TADG-14 KLK9 KLK-L3 protein KLK10 NES1 protein KLK11 TLSP/hippostasin KLK12 KLK-L5 protein KLK13 KLK-L4 protein KLK14 KLK-L6 protein New gene GenBank symbol (a,b) Accession No.