8] Human genes: KLK3, kallikrein-related peptidase 3; TERT, telomerase reverse transcriptase; FGFR2, fibroblast growth factor receptor 2; TBBX3, T-box protein 3; KLK15, kallikrein-related peptidase 15; KLK2, kallikrein-related peptidase 2; KLK1, kallikrein-related peptidase 1; ACPT, acid phosphatase, testicular; GPR32, G protein-coupled receptor 32; INS, insulin; KLK4
, kallikrein-related peptidase 4; KLK9, kallikrein-related peptidase 9; KLK10, kallikrein-related peptidase 10; KLK14, kallikrein-related peptidase 14.
Low concentrations of KLK4 were found in a wide variety of tissues, adult and fetal.
KLK4 was primarily found in seminal plasma, with lower concentrations in breast milk and urine.
6] Human genes: KLKI, kallikrein 1, renal/pancreas/salivary; KLK2, kallikrein 2, prostatic; KLK3, kallikrein 3 (prostate-specific antigen); and KLK4
, kallikrein 4 (prostase, enamel matrix, prostate).
All of the above studies have examined total KLK4 mRNA expression.
In contrast to the multiple studies examining KLK4 at the mRNA level, only 3 published studies have investigated the KLK4 gene product (the hK4 protein).
AJ310938) and KLK4 variant 1 for the KLK4 gene (GenBank accession no.
AJ310938) and KLK4 variant 1 (AF148532), we designed primers that bound in coding exons 3 and 5 (F1 and R1 primers, respectively; Fig.
KLK7 is up-regulated in ovarian cancer (62), and KLK4 and KLK5 are indicators of poor prognosis of ovarian cancer (63-65).
An alternatively spliced variant of KLK4 expressed in prostatic tissue.
Because the open reading frame of KLK4
predicts that its first 26 N-terminal amino acids are the signal peptide, we expected that the 224-amino acid mature enzymatic form would be a secreted protein (3, 5, 9).
Localization of a new prostate-specific antigen-related serine protease gene, KLK4
, is evidence for an expended human kallikrein gene family cluster on chromosome 19813.