Inhibitor and activator combinations were used to assess the contribution of the different PPases to RIIp dephosphorylation in Jurkat cell
Human Jurkat cells
are more sensitive to the recombinant leucine zipper--TRAIL (15), which induces the apoptosis of human breast cancer MDA-231 cells without harming normal breast epithelium cells and other normal tissues.
The experiment involved normal peripheral blood mononuclear cells (PBMCs) as well as the T-cell leukemia Jurkat cell
Exposure of human p53 mutant Jurkat cells
to DNA-damaging agents activates arrest in the G2 cell cycle phase at the G2/M DNA damage checkpoint and attempts homologous recombination repair (Seifrtova et al.
In a separate experiment, Jurkat cells
were treated with 1 mg/L PHA for 12 h as a positive control for apoptosis induction.
3G4 also significantly enhanced macrophage phagocytosis of apoptotic Jurkat cells
INFLUENCE OF GLUCOSE, FRUCTOSE, VANADIUM AND THEIR COMBINATIONS ON METABOLIC TOXICITY AND SURVIVAL OF JURKAT CELLS
Key words: apoptosis, Jurkat cells
, mitogen-activated protein kinases, pentachlorophenol, tetrachlorohydroquinone.
Interestingly, they found that Jurkat cells
did not develop stress granules in response to darinaparsin treatment and this cell line showed accelerated toxicity compared with THP1 and Oci cells, which did form stress granules.
1] and allowed to adhere in 96 well plates for a minimum of 4 h In parallel U937 and Jurkat cells
were also seeded and different CPT extracts were added to all the cells at the same time and they were cultured further for 48 h.
The mechanism of inhibition involved selective induction of apoptosis in liver-infiltrating T lymphocytes, activated spleen T cells, and activated Jurkat cells
, without affecting hepatocytes, naive spleen T cells, nonactivated Jurkat cells
, or other tissue cells (7, 8).
Results indicate that IL-i3 promoter activity is highly inducible by calcium and PKC signals in Jurkat cells