isoelectric focusing (redirected from Isoelectric focussing)

focusing /fo·cus·ing/ (-ing) the act of converging at a point.

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isoelectric focusing  electrophoresis in which the protein mixture is subjected to an electric field in a gel medium in which a pH gradient has been established; each protein then migrates until it reaches the site at which the pH is equal to its isoelectric point.

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isoelectric focusing,

the ordering and concentration of substances according to their isoelectric points.

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focusing [fo´kus-ing]

the act of converging at a point.

isoelectric focusing electrophoresis in which the protein mixture is subjected to an electric field in a gel medium in which a pH gradient has been established; each protein then migrates until it reaches the site at which the pH is equal to its isoelectric point.

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isoelectric focusing,

n the ordering and concentration of substances according to their isoelectric points.

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focusing

the act of converging at a point.

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isoelectric focusing

electrophoresis in which the protein mixture is subjected to an electric field in a gel medium in which a pH gradient has been established; each protein then migrates until it reaches the site at which the pH is equal to its isoelectric point.

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isoelectric

showing no variation in electric potential.

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isoelectric focusing

an electrophoretic method for separating proteins in gels which depends on the fact that the net charge on the protein varies with the pH of the surrounding medium. At the isoelectric point the protein has no net charge and will not therefore migrate further in an electric field. The polyacrylamide gel is cast in a narrow tube that provides a pH gradient from top to bottom.

isoelectric period

the moment in muscular contraction when no deflection of the galvanometer is produced.