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insertion sequence |
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Microarray data that indicated the presence of a deletion were verified by PCR analysis, which used primer pairs that spanned the insertion sequences of the respective plasmids, the flanking regions, or both. In fact, this phenomenon was demonstrated for the GR-541 plasmid that contains multiple copies of insertion sequences and other mobile genetic elements within its scaffold (14). Moreover, in some species, rapid genomic rearrangements occur because of repeats or insertion sequences, so even if isolates descended from a common ancestor that arose several decades ago, they may not readily be seen to be minor variants of the same clone. |
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