To test the antigen recognized by MAB 1H12 for cross-reactivity with antibodies specific for neural cell adhesion molecule (NCAM) and the carbohydrate epitope HNK-1, immunoprecipitated
antigen was electrophoresed and transferred to nitrocellulose as described above.
Briefly, nuclear lysates isolated from MDBK cells were sonicated, and cross-linked proteins were immunoprecipitated
by incubation with antibody against Sp1 (Santa Cruz Biotechology Inc.
The Ct values of ER[alpha] antibody-precipitated DNA were adjusted by nonspecific immunoprecipitated
acetyl-p53 levels were measured on immunoprecipitated
The enzymatic activity of immunoprecipitated
phosphorylated Ala was then assessed in vitro using the supplied glycogen synthase kinase (GSK)-3[alpha]/[beta] fusion protein as substrate, in the presence of ATP.
In this technique phages expressing candidate autoantigens are immunoprecipitated
with antibodies in the patient sample and identified by high-throughput DNA sequencing (19).
Sheared chromatin is then immunoprecipitated
with DNA topo II or control antibodies.
While patient serum was not reactive with membrane fraction of CHO/IR cells on Western blots, the hIR from CHO/IR, the serum immunoprecipitated
the hIR from CHO/IR membrane fragments such that IR was detected with commercial Abs insulin receptor-alpha and insulin receptor-beta subunits.
After performing cell lysis and fractionation yielding Triton-soluble and Triton-insoluble fractions, we immunoprecipitated
both fractions with an anti-FAK antibody.
Equal amounts of protein (1 mg) from 2 endometrial carcinomas and 1 endometrial polyp were immunoprecipitated
overnight at 4 [degrees] C with either 1 [micro]g c-Kit monoclonal antibody (clone K45) or saline, and then incubated with protein A/G PLUS agarose (Santa Cruz Biotechnology, Santa Cruz, Calif) for 1 hour at 4 [degrees] C.
DNA-PK holoenzyme was immunoprecipitated
with PAS beads that had been coated with the Ku antibody as follows: PAS beads were pre-swollen in kinase assay buffer (50 mM HEPES, pH 7.
The paper entitled "Chromatin Profiling by Directly Sequencing Small Quantities of Immunoprecipitated
DNA" appeared in the online version of Nature Methods yesterday.