immunoprecipitation

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Related to Immunoprecipitated: Coimmunoprecipitation

immunoprecipitation

 [im″u-no-pre-sip″ĭ-ta´shun]
precipitation resulting from interaction of specific antibody and antigen.

im·mu·no·pre·cip·i·ta·tion

(im'yū-nō-prē-sip'i-tā'shŭn),
The phenomenon of aggregation of sensitized antigen on addition of specific antibody (precipitin) to antigen in solution.

immunoprecipitation

/im·mu·no·pre·cip·i·ta·tion/ (-pre-sip″ĭ-ta´shun) precipitation resulting from interaction of specific antibody and antigen.

immunoprecipitation

[-prisip′itā′shən]
a procedure used to isolate target molecules with which antibodies react. Precipitation results when insoluble antigen-antibody complexes are formed by the reaction between soluble antigens and antibodies.

immunoprecipitation

precipitation resulting from interaction of antibody and soluble antigen.
References in periodicals archive ?
To test the antigen recognized by MAB 1H12 for cross-reactivity with antibodies specific for neural cell adhesion molecule (NCAM) and the carbohydrate epitope HNK-1, immunoprecipitated antigen was electrophoresed and transferred to nitrocellulose as described above.
Briefly, nuclear lysates isolated from MDBK cells were sonicated, and cross-linked proteins were immunoprecipitated by incubation with antibody against Sp1 (Santa Cruz Biotechology Inc.
The Ct values of ER[alpha] antibody-precipitated DNA were adjusted by nonspecific immunoprecipitated DNA.
The enzymatic activity of immunoprecipitated phosphorylated Ala was then assessed in vitro using the supplied glycogen synthase kinase (GSK)-3[alpha]/[beta] fusion protein as substrate, in the presence of ATP.
In this technique phages expressing candidate autoantigens are immunoprecipitated with antibodies in the patient sample and identified by high-throughput DNA sequencing (19).
Sheared chromatin is then immunoprecipitated with DNA topo II or control antibodies.
While patient serum was not reactive with membrane fraction of CHO/IR cells on Western blots, the hIR from CHO/IR, the serum immunoprecipitated the hIR from CHO/IR membrane fragments such that IR was detected with commercial Abs insulin receptor-alpha and insulin receptor-beta subunits.
After performing cell lysis and fractionation yielding Triton-soluble and Triton-insoluble fractions, we immunoprecipitated both fractions with an anti-FAK antibody.
Equal amounts of protein (1 mg) from 2 endometrial carcinomas and 1 endometrial polyp were immunoprecipitated overnight at 4 [degrees] C with either 1 [micro]g c-Kit monoclonal antibody (clone K45) or saline, and then incubated with protein A/G PLUS agarose (Santa Cruz Biotechnology, Santa Cruz, Calif) for 1 hour at 4 [degrees] C.
DNA-PK holoenzyme was immunoprecipitated with PAS beads that had been coated with the Ku antibody as follows: PAS beads were pre-swollen in kinase assay buffer (50 mM HEPES, pH 7.
The paper entitled "Chromatin Profiling by Directly Sequencing Small Quantities of Immunoprecipitated DNA" appeared in the online version of Nature Methods yesterday.