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immunoprecipitation
(redirected from Immunoprecipitated)

   Also found in: Encyclopedia, Wikipedia 0.01 sec.
immunoprecipitation /im·mu·no·pre·cip·i·ta·tion/ (-pre-sip″ĭ-ta´shun) precipitation resulting from interaction of specific antibody and antigen.
im·mu·no·pre·cip·i·ta·tion (my-n-pr-sp-tshn, -my-)
n.
The precipitation of sensitized antigen as the result of the interaction of antigen with a specific antibody in solution.

immunoprecipitation
[-prisip′itā′shən]
a procedure used to isolate target molecules with which antibodies react. Precipitation results when insoluble antigen-antibody complexes are formed by the reaction between soluble antigens and antibodies.

immunoprecipitation [im″u-no-pre-sip″ĭ-ta´shun]
precipitation resulting from interaction of specific antibody and antigen.

immunoprecipitation
precipitation resulting from interaction of antibody and soluble antigen.


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After lysing cells with cold TBS containing 1% TritonX-100 and protease inhibitor cocktail (Roche) followed by centrifugation, the supernatants were immunoprecipitated with anti-FLAG M2 antibody (Sigma).
Immunoprecipitated DNA samples were then set to real-time PCR analysis to quantify the relative amount to their corresponding input controls with a LightCycler (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer's instructions.
Strips of 21-nucleotide-long DNA subsequently are released from the immunoprecipitated DNA to create "genomic signature tags," which subsequently are identified in the international genome database.
 
 
 
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