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immunoblotting |
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immunoblotting [-blot′ing] a method for identifying antigens. The antigens are allowed to adhere to cellulose sheets and are identified by staining with labeled antibodies. The method is also used to detect monoclonal proteins. See also Western blot test. immunoblotting, n the immunologic methods for isolating and quantitatively measuring immunoreactive substances. When used with immune reagents such as monoclonal antibodies, the process is known generically as Western blot analysis. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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Comparison of lipopolysaccharide (LPS) antigens from the 2 strains by sodium dodecyl sulfate--polyacrylamide gel electrophoresis and immunoblotting showed that they differed in O-repeating units (Figure, panel B). Similar amounts of wild-type and mutant AQP9s were detected in the yeast membranes by immunoblotting (data not shown). We performed immunoblotting using polyclonal rabbit antibodies against either GLUT 1 (lot A970212)([parallel][parallel]) or GLUT 4 protein (RALRGT, lot 819/4299). |
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