The diagnostic discriminator, assuming that generation of Ara-CTP in AML cells is the major cause of cell death (4, 5), is the maximal difference in light output between the IPTG
(LI) and IPTG
+ AP (LIP) curves that are shown in Fig.
Detectable amounts of the analytes were observed in the absence of induction, and the analytes varied widely with regard to the amount that accumulated in these conditions, but this "leaky" expression did not significantly inhibit growth before IPTG
We look forward to continuing our growth and success by offering our solutions in conjunction with Open Solutions IPTG
In this paper, the researchers used the system to detect light, a lactose metabolite called IPTG
, and an antibiotic derivative called aTc, but it could be tailored to many other molecules or even signals produced by the cell, Lu says.
Co-expression of human CYP1A2 protein and NADPH-CYP reductase was induced by IPTG
CUZD1 production was induced by IPTG
(isopropylthio-j8-galactoside) induction, as previously described (11).
According to these cases at the continuances of zymogene fermentation of saccharomyces cerevisiae with AAT Gen and the starting of inspiration during 3/4/5/6/7 hours after insemination with IPTG
The transformed cells were spread on LB agar plates containing X-gal (300 ug/mL), IPTG
(120 ug/mL) and ampicillin (100 ug/mL).
coli Expression Systems including, Sugar Inducible, Depletion Inducible, Optimized IPTG
Inducible Platforms, and Lonza s pDNA Services Platform.
0, and induced with 60 [micro]M IPTG
(isopropyl-1-thio-,3-D-galactoside) for 3 h at 37[degrees]C.
The proteins (NT-gp96 and CT-gp96) were induced individually using 1 mM IPTG
Autoclave to sterilize; cool to around 50[degrees]C; add appropriate antibiotics and other supplements, such as X-Gal and IPTG
, if needed; and then pour).