Therefore, to understand the expression and regulation of PG transporters in porcine uterine endometrium during the estrous cycle and pregnancy, we evaluated i) expression of ABCC1, ABCC9, SLCO4C1, and SLCO5A1 mRNAs in the uterine endometrium during the estrous cycle and pregnancy, in the conceptus of early pregnancy, and in chorioallantoic tissues during pregnancy; ii) localization of ABCC1 and ABCC9 mRNAs in the uterine endometrium; and iii) effects of steroid hormones, IL1B
, and interferon gamma (IFNG) on ABCC1, ABCC9, SLCO4C1, and SLCO5A1 mRNA expression in the endometrial tissues.
These genes encoded cytokines (CSF3, CSF2, CCL3, TNF, CCR1, IL13, KIT, CCL5, CCL4, IL10, CXCL10, IL12RB2, IFNG, IL1B
, IL1A, IL8, MET, CD40, HGF, LEP, CXCL16, CX3CR1, CCR2, PDGFRA, IL12B, IL2), lipid metabolism related genes (CD36, GPX4, LPIN1, LPL, LPB), transcription regulators (BCL3, FOS and NFKBIA), receptors (TNF, IL8, RELA, TLR1, TIRAP, TLR2, NFKBIA, NFKB1, TLR4, CD40, CCL5, CXCL10, FOS, JUN, MAP3K8, IL1B
, LBP, IL12B, CD14, SPP1), and others such as SELP, SELL, and SOD1, all play a role in some aspect of the immune response including cytokine activity (IL10, TNF, IL8, and IL1B
), cell adhesion (SELL and SELP), immune activation (CD14 and TLR2), acute phase reaction (TNF, IL1B
, and SAA3), apoptosis (BCL2, BAX).