These molecules include proinflammatory cytokines, such as IL-1, IL-6 and alpha tumor necrosis factor (TNF-[alpha]), clonal expansion and IL-2, subset of auxiliary T lymphocytes (Th1), IL-12 and gamma interferon (IFN-[gamma]) and, to a lesser extent, the Th2, IL-4 and IL-5 subset, colony-stimulating factors such as granulocyte and macrophage (GM-CSF), chemokines such as RANTES and MIP-1a, adhesion molecules such as ICAM1
, ELAM-1 and E-selectin, inflammatory mediators such as bradykinin, histamine, eicosanoids and nitric oxide, as well as molecules involved in the presentation of antigen and class II major histocompatibility complex (MHC).
RT-PCR was performed for ICAM1, VCAM1, CCL2, BCL2, BAX and CASP9.
Effects of EGCG and L-theanine on TNF-[alpha]-induced ICAM1, CCL2 and VCAM1 gene expression in cultured endothelial cells
05) decreased by the addition of 10 [micro]M or 30 [micro]M EGCG, with 30 [micro]M EGCG almost completely inhibiting increases in ICAM1 gene expression induced by TNF-[alpha].
05) upregulated expression of ICAM1, CCL2 and VCAMI genes, and this effect could also be reduced by treatment with EGCG and L-theanine (Fig.
We selected 18 candidate genes related to As metabolism (PNP, GSTO1, GSTM1, GSTP1, GSTT1, AS3MT, MTHFR, and CBS), oxidative stress (HMOX1, NOS3, SOD2, and CYBA), and inflammation/endothelial dysfunction (APOE, TNF, IL6, ICAM1, VCAM1, and S1PR1).
The multiplicative interaction between well-water As and 2 SNPs--rs281432 in ICAM1 ([p.
The joint effects for the ICAM1 rs281432 and VCAM1 rs3176867 SNPs considered with dichotomous well-water As are presented in Table 3.
2]-isoprostanes A, B P-selectin A, B Soluble ICAM1
A, B (a) A, Variable investigated in relation to year 0 carotenoids.
The level of transcripts encoding CYP19A1, FABP4, ICAM1, IGFBP3, IL1B, TRIB3, or VCANI1 was modulated only in precursors.
The effect of PCB-153 in precursors was similar to that of AhR ligands for CYP19A1, ICAM1, PTGS2, TNFRSF11B, and VCAM1 and different for CXCL12, MB, IGFBP3, NPTX1, STAT1, THBS1, and WNT5A.
To validate this results, we assessed the modulation of ICAM1 (Brake et al.