Diatrizoate, see there.
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Posteriormente, se centrifugo la muestra para obtener el plasma y la fraccion celular, a partir de la cual se separaron celulas mononucleares de sangre periferica, utilizando el gradiente de densidad de Ficoll Hypaque (GE Healthcare Bio-Sciences Corp.
Neutrophils were isolated using a standard method by dextran sedimentation and centrifugation in a Ficoll Hypaque gradient (Boyum 1968).
Separation of MNCs: Mononuclear cells (MNCs) were isolated from adult human peripheral blood by gradient centrifugation at 2500 rpm for 30 minutes on Ficoll Hypaque (Biosera, France).
Stromal Vascular Fraction (SVF) was isolated and cultured in DMEM culture medium (GIBCO) containing 10% fetal bovine serum (GIBCO) using Ficoll hypaque (Biosera) gradiant centrifugation.
peripheral blood mononuclear cells (PBMC) Heparinized venous blood samples were obtained from patients and controls, and PBMC were isolated using the Ficoll Hypaque density-gradient centrifugation technique.
Radigraphic detection of accesory/laterals canals: use of radiovisiography and hypaque.
Trypanosoma cruzi: separation of broad and slender trypomastigotes using a continuous hypaque gradient.
Density gradient selection using Ficoll Hypaque (FH)-based gradients remains the most common approach.
Next, the blood was layered over 9 ml Ficoll Hypaque density gradient (LSM 1077, PAA, Laboratories GmbH, Austria) and centrifuged (1800 rpm, 20 min, 4[degrees]C).
Berlyne, "Acute renal failure following intravenous pyelography with hypaque," Acta Medica Scandinavica, vol.
From the l0 ml samples the plasma and peripheral blood mononuclear cells (PBMC) were separated by the density gradient technique using Ficoll hypaque and stored in multiple aliquots at -60[degrees]C until tested.