After washing with PBS, cells were stained with haematoxylin
and Eosin In: Theory and practice of histological techniques (J.
According to GOODWIN (1989), the haematoxylin
and eosin-staining of tissue sections is a sensitive method for the identification of the parasites.
The counterstaining was performed with Harry's haematoxylin
Paraffinated blocks were further processed in order to prepare 5 micron sections using microtome which then were stained by haematoxylin
(Harris modified haematoxylin
with acetic acid; Fisher Scientific, Pittsburgh, PA, USA) and eosin (10% in ethanol, Electron Microscopy Science, Pittsburgh, PA, USA) staining method and observed by light microscope.
and eosin stained paraffin sections of the mass on low power showed tumor cells to infiltrate in solid sheets (Figure 2a).
Also, we fixed tissue of the nodule in a 10% neutral-buffed formalin, processed routinely, embedded in paraffin wax, sectioned at 4-6 mm and stained with haematoxylin
and eosin (H&E) and Masson's trichrome methods.
Neuronal injury in the cortex was measured by staining sections with haematoxylin
and eosin using the standard method (8).
They were then transferred to haematoxylin
solution for 10 min, washed under running tap water for 10 min, and finally transferred to eosin solution for 5 min before redehydration by passage through 90[degrees] alcohol (2 x 1 min), 100[degrees] alcohol (2 x 1 min) and xylol (2 x 5 min).
These fibres are difficult to detect with haematoxylin
and eosin staining especially during frozen sections.
These tissues were fixed in natural buffered formalin and then processed by using paraffin embedding technique for preparation of sections and stained with haematoxylin
and eosin (H and E) for observing microscopic changes caused by M.
Histopathological examinations: Histopathological analysis was performed on primary tumours on haematoxylin
and eosin stained sections.