We used sandwich ELISA methods to measure H-ficolin, L-ficolin, and mannose-binding lectin (MBL) concentrations in plasma samples.
After blocking the endogenous peroxidase with 30 g/L hydrogen peroxide, for ficolin staining alone, sections were incubated in TBS with normal sheep serum, with either H-ficolin monoclonal antibody (clone 4H5) from HyCult Biotechnology or rabbit polyclonal anti-L-ficolin antibody (clone 2F5) from Tokai University.
By tryptic peptide fingerprinting analysis, we confirmed that the identities of the 2 peptides were Hakata antigen and hucolin, known as H-ficolin and L-ficolin, respectively (Fig.
We detected a 4- to 5-fold increase in the concentrations of both H-ficolin and L-ficolin in maternal plasma of normal pregnancies (n = 45; Fig.
We confirmed that maternal plasma concentrations of H-ficolin and L-ficolin were significantly lower in preeclamptic pregnancies (n = 20) than in uncomplicated pregnancies (Fig.
To understand the role of ficolins in the underlying immune recognition in preeclampsia, we revealed high concentrations of H-ficolin and L-ficolin in placental lysate from established preeclamptic pregnancies (n = 5; Fig.
Hiroshi Shiraki for H-ficolin, L-ficolin, MBL antibodies/ antigens and the polysaccharide from Aerococcus viridans; Dr.
Activation of the lectin complement pathway by H-ficolin (Hakata antigen).