Cyclodextrins are naturally occurring oligosaccharides consisting of D-glucose molecules linked end-to-end by [alpha]-1,4 glycosidic bonds
to form a circular structure.
In contrast to cellulose, the alpha-anomer--the alpha-1,4 glycosidic bonds
between glucose units--prefers a helical assembly, as seen in starch.
Chitin is a biopolymer composed of N-acetyl D-glucosamine units linked by [beta]-1, 4 glycosidic bonds
Pectinase is a generic name for a family of enzymes that catalyse hydrolysis of the glycosidic bonds in the pectic polymers .
Which showed that the process of hydrolyzing the internal glycosidic bonds was accomplished faster which necessitated the formation less complex substrates for further hydrolysis by exo pectinases.
Polysaccharide may be degraded/fragmented through cleavage of inter-subunit glycosidic bonds
by chemical reactions that often involve simple but randomly-acting acid or reactive oxygen species, or biological reactions that often involve complex but specific enzymes [1, 2].
The pectin is obtained as a linear polymer of D-galacturonic acid and its methyl ester in which the monomer units are linked by 1, 4 glycosidic bonds
It's an extremely large molecule composed of many repeating units of the disaccharide, D-N-acetylglucosamine, and D-glucuronic acid, linked together via alternating beta-1,4 and beta-1,3 glycosidic bonds
Its enzyme activity is rated at 96 AZ per gram (an AZ is the degree of activity that catalyzes splitting of a number of glycosidic bonds
equivalent to 0.
Among the depolymerases, polygalacturonase is the major enzyme with a hydrolytic function and lyases (or transeleminases) which cleaves glycosidic bonds
forming unsaturated product[DELTA](4,5-D-galacturonate) through transelimination reaction .
The dual activity of [beta]-glucosidase leading to cleavage and synthesis of glycosidic bonds
is pivotal in many crucial biological pathways such as cellular signaling, biosynthesis and degradation of structural and storage polysaccharides, host-pathogen interactions, as well as in a number of biotechnological applications .
We know that polysaccharides are carbohydrates containing thousands of glucose units joined by alpha or beta glycosidic bonds