Giemsa stain


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stain

 [stān]
1. a substance used to impart color to tissues or cells, to facilitate microscopic study and identification.
2. an area of discoloration of the skin.
acid-fast stain a staining procedure for demonstrating acid-fast microorganisms.
differential stain one that facilitates differentiation of various elements in a specimen.
endogenous stain an intrinsic stain acquired during tooth development.
exogenous stain an intrinsic stain acquired after a tooth has erupted.
extrinsic stain a stain that can be removed from a tooth surface by polishing.
Giemsa stain a solution containing azure II-eosin, azure II-glycerin, and methanol; used for staining protozoan parasites such as Plasmodium and Trypanosoma, for Chlamydia, for differential staining of blood smears, and for viral inclusion bodies. Stained elements appear pink to purple to blue.
Gram stain a staining procedure in which bacteria are stained with crystal violet, treated with strong iodine solution, decolorized with ethanol or ethanol-acetone, and counterstained with a contrasting dye; those retaining the stain are called gram-positive, and those losing the stain but staining with the counterstain are called gram-negative.
hematoxylin and eosin stain a mixture of hematoxylin in distilled water and aqueous eosin solution, employed universally for routine examination of tissues.
intrinsic stain a stain that is within the enamel of a tooth and cannot be removed by polishing.
metachromatic stain one that produces in certain elements a color different from that of the stain itself.
nuclear stain one that selectively stains cell nuclei, generally a basic stain.
port-wine stain a persistent dark red to purple nevus flammeus that grows proportionately with the affected child and is usually found on the face. Initially it is macular, but the surface may develop angiomatous overgrowths with time. Port-wine stains often occur in association with other congenital abnormalities.
supravital stain a stain introduced in living tissue or cells that have been removed from the body.
tumor stain an area of increased density in a radiograph, due to collection of contrast material in distorted and abnormal vessels, prominent in the capillary and venous phases of arteriography, and presumed to indicate neoplasm.
vital stain a stain introduced into the living organism, and taken up selectively by various tissue or cellular elements.
Wright's stain a mixture of eosin and methylene blue, used for demonstrating blood cells and malarial parasites.

Giem·sa stain

(gēm'să),
compound of methylene blue-eosin and methylene blue used for demonstrating Negri bodies, Tunga species, spirochetes and protozoans, and differential staining of blood smears; also used for chromosomes, sometimes after hydrolyzing the cytologic preparation in hot hydrochloric acid, and for showing chromosome G bands; often used in glycerol-methanol buffer solution.

Giem·sa stain

(gēm'să stān)
Compound of methylene blue-eosin and methylene blue used for demonstrating Negri bodies, Tunga species, spirochetes and protozoans, and differential staining of blood smears; also used for chromosomes, sometimes after hydrolyzing the cytologic preparation in hot hydrochloric acid, and for showing chromosome G bands.

Giemsa,

Gustav, German bacteriologist, 1867-1948.
Giemsa chromosome banding stain - a unique chromosome staining technique used in human cytogenetics to identify individual chromosomes. Synonym(s): G-banding stain
Giemsa stain - compound used for demonstrating Negri bodies, Tunga species, spirochetes and protozoans, and differential staining of blood smears.

Giemsa stain

a solution containing azure II-eosin, azure II, glycerin and methanol; used for staining protozoan parasites, Leptospira, Borrelia, viral inclusion bodies and Rickettsia.
References in periodicals archive ?
B and C, Original slides were not retained, but representative cytology with modified Giemsa stain shows ductal carcinoma (original magnifications 310 [B] and 340 [C]).
Hematopoietic cells stained by Giemsa stain, Immunohistochemistry or ISH (with bright field and fluorescent) prepared from cell suspension
Inflammatory cells can be easily detected with the used of giemsa stain (Figure 2).
Patients' blood samples were collected and a rapid stain (10% Giemsa stain for 10 min) was done to screen patients for full enrollment.
Turk's solution preparation: Dilute 3 mL glacial acetic acid to 100 mL total volume with deionized water and add two to three drops methylene blue or Giemsa stain.
The sections were stained using a modified Giemsa stain containing 25 ml Giemsa stock solution ("Merck", Germany, product 1.
68-23-5) (17a)-17- Hydroxy-19-norpregn-5(10)en-20-yn-3-one (Sigma); R PMI 1640, Fetal calf serum, Phytohaemagglutinin-M, Antibiotic-antimycotic mixture (Gibco); Dimethylsulphoxide, Colchicine, Giemsa Stain (Merck); Hoechst 33258 (Sigma); 5-Bromo-2-deoxyuridine, Ascorbic acid (SRL, India).
Slides were treated with trypsin and Giemsa stain for G-band analysis following the method of Baker et al.
Tnder are invited for Supply of vaious chemicals CYtochrome Leishman Stain with Buffer, Bendedict Reagent, Paraffin Wax (60A-6,2AC) Giemsa Stain Soln, Isopropy Aocoho,l Conc.