05 M buffer of N-(2-Acetamido)-2-aminoethanesulfonic acid (ACES) at pH 7 were inoculated with two different spore strains--Bacillus amyloliquefaciens and Geobacillus stearothermophilus
spores use: sterilization, state: liquid, geobacillus stearothermophilus
: 10,000,000 colony forming units / vial methodology: microbiology, biological indicator: especially for sterilizing liquid,glycerol concentration: 87% usage: laboratory: liquid.
Conditions for Sterilization of the Most Commonly Encountered Devices in the Dental Office Method Temperature Time ([degrees]C/ (minutes) [degrees]F) Steam autoclave Gravity displacement 30 121/250 Pre-vacuum sterilizer 4 132/270 Dry heat Static air 60 170/340 120 160/320 150 150/300 Forced air 12 190/375 Unsaturated chemical 20 132/270 Modified from references 1-3,5,6 Table III: Biological Monitoring Indicated for Various Sterilizers Method Biological Monitoring (Spore Used) Steam autoclave Gravity Geobacillus stearothermophilus
displacement (Formerly B.
The researchers deposited spore preparations of Bacillus subtilis and Geobacillus stearothermophilus
onto the surface of pyrex microscope slides at about [10.
Gene cloning, expression, and crystallization of a thermostable exo-inulinase from Geobacillus stearothermophilus KP1 289.
awamori , Geobacillus stearothermophilus KP1289 , Arthrobacter sp.
Scientists at Clemson University tested spores of Geobacillus stearothermophilus
as a surrogate bacterial strain in rendering materials at various times and temperatures, simulating the commercial rendering process.
The sensitivity of Geobacillus stearothermophilus
to MB, for example, would lead to a possible assumption of total clearing that may not exist, while the increased resistance seen in Bacillus thuringiensis would lead to an assumption of continued contamination when no contamination exists.
On the one hand, the sensitivity of Geobacillus stearothermophilus
to MB would lead to a possible assumption of total clearing that may not exist.
ProTest Steam is a self-contained biological indicator inoculated with viable Geobacillus stearothermophilus
bacterial spore, and is intended for monitoring the efficacy of steam sterilization cycles (121[degrees]C gravity displacement and 121[degrees]C to 135[degrees]C pre-vacuum cycles).
An independent testing laboratory is consistently achieving 100% kill of Geobacillus stearothermophilus
, the standard spore used for testing surgical instrument sterilizers.
Is Geobacillus stearothermophilus
a good surrogate for Bacillus anthracis during methyl bromide fumigation?