knockdown

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Related to Gene knockdown: Gene knockout
The alteration of a gene’s function so that it is conditionally expressed, a manoeuvre required when complete gene knockout would be lethal to the organism

knockdown

A modification of a gene that limits its expression to specified conditions or circumstances.

knockdown

a measure of biocide effect that is used when it is difficult to assess actual mortality. The measure is used particularly in biocide tests on insects, and has been shown to produce similar results to actual mortality scores. Knockdown is often measured as the inability to respond to a stimulus such as light or touch. see KNOCKDOWN LINE.
References in periodicals archive ?
For the initial validation of plasmid and siRNA transfection, culture containing serum was used while rest of the experiments on CYP19 gene knockdown was done in serum free culture.
The poster demonstrates that SurModics' Eureka(TM) DUET polymer platform can be used for sustained release of cationic lipid/siRNA complexes which retain high levels of gene knockdown activity.
Here at Vanderbilt, many researchers are interested in targeted gene knockdown, and access to the MISSION(TM) TRC shRNA Library will greatly accelerate their ability to prioritize genes identified from various proteomic and genomic screens.
This visionary platform has enormous potential to combine into one nanoparticle all possible RNA functionalities including receptor antagonism/activation, cell targeting/delivery and gene knockdown through mechanisms like RNA interference.
Our initial characterization of these reagents leads us to believe that this RNAi knockdown platform will be widely useful for cancer target discovery and verification, and that the lentiviral backbone and technological support provided by Sigma-Aldrich make for rapid and easy adaptation of these reagents to a wide variety of gene knockdown experiments carried out by basic and translational investigators at the Moores UCSD Cancer Center.
The scientists addressed several issues for the use of RNAi as a screening method, including the efficient gene transfer, the specificity and efficiency of the gene knockdown, and other technical requirements.
When used together, QuantiGene bDNA assays and SMARTpool siRNA products will allow researchers to accurately and efficiently validate RNAi gene knockdown studies, as well as compare and standardize data between experiments and laboratories.