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GSSG |
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Glutathione peroxidases (GPx) were measured at 340 nm in a coupled enzyme system where cumene hydroperoxide is used as substrate for the sum of Se-dependent and Se-independent forms and NADPH is consumed by GR to convert the formed GSSG to its reduced form. For total GSH, GSH was oxidized by DTNB to give GSSG with stoichiometric formation of 5-thio-2-nitrobenzoic acid (TNB). We calculated the amount of total glutathione and GSSG in the samples from the standard curves. |
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