References in periodicals archive ?
GSSG was determined by direct derivation of GSH, by homogenizing another sample of the same tissue in the presence of 12.
3] induces formation of GSSG, which is then detected by the roGFP2 probe, we asked whether glutathione peroxidases (GPx), major generators of GSSG, are involved in the [O.
The present study evaluates the changes in glutathione redox ratio expressed as GSSG x [GSH.
When GSSG accumulates in cells, it can undergo disulfide exchange reactions with protein thiols, leading to their S-glutathionylation.
We also showed that reduced GR levels leads to an increase in GSSG levels which contribute in elevation of arsenic toxicity in guinea pigs (96).
We evaluated recovery by adding known concentrations of GSH or GSSG (400 [micro]mol/L) to whole blood from a healthy donor (N3).
Samples for GSSG measurement were immediately mixed with thiol-scavenging reagent M2VP (l-methyl-2-vinyl-pyridium trifluoromethane sulfonate) after separation.
Conversely, GSSG levels were significantly high in aged rats.
If GSSG accumulates within the cell, it can create protein-glutathione adducts via thiol-disulfide exchange reactions.
Recently, research has increasingly recognized that the amounts of GSH and GSSG and the ratio of GSH to GSSG are reliable indices of redox status and the extent of oxidative stress.
GSH and GSSG levels were normalized to protein concentrations and the GSH/GSSG ratio was calculated.
Table 1 summarizes some of the results obtained by different investigators for the concentrations of GSH and GSSG in blood samples: the extremely high variability of GSH and, moreover, of GSSG is evident.
Medical browser ?
- Grutz, Otto
- Grynfeltt triangle
- Grynfeltt, Joseph C.
- Grzybowski, M.
- gs antigen
- G-series nerve agents
- gSowa rigpa
- GTP cyclohydrolase
- GU71u344 virus
- GU71u350 virus
- gua sha
- guaiac test
Full browser ?
- GSS API
- GSS syndrome