The slides were stained with primary antibody against GLUT-1 (1:200, polyclonal, Dako).
GLUT-1 is preferentially expressed in atypical endometrial hyperplasia and endometrial adenocarcinomas.
Clinicopathological significance and linkage of the distribution of HIF-1 alpha and GLUT-1 in human primary colorectal cancer.
Kunkel M, Moergel M, Stockinger M, Jeong JH, Fritz G, Lehr HA, et al Overexpression of GLUT-1 is associated with resistance to radiotherapy and adverse prognosis in squamous cell carcinoma of the oral cavity.
4,5] Peripheral nerves, which like the brain depend heavily on glucose for metabolism, have a blood-nerve barrier in which GLUT-1 also plays a major role, and the perineurium of these fibers has been demonstrated to stain intensely with antibodies to GLUT-1 by immunohistochemical techniques.
As GLUT-1 antibodies enable the perineurium to be identified in both large and small nerve fibers, we studied material from healthy and HD tissues to assess the distribution of perineurial-bound nerve fibers and to determine if their demonstration has diagnostic usefulness.
3% hydrogen peroxide to block endogenous peroxidase and then incubated with 5% normal rabbit serum (for NGFR staining) or goat serum (for GLUT-1 staining) to minimize nonspecific reaction, followed by rinsing in phosphate-buffered saline.
After thorough rinsing in phosphate-buffered saline, biotinylated rabbit anti-mouse immunoglobulin (1:400, Dakopatts, Glostrup, Denmark) for NGFR staining and goat anti-rabbit immunoglobulin (1:400, Dakopatts) for GLUT-1 staining were applied for 60 minutes at room temperature.