GC content


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GC con·tent

the amount of guanine and cytosine in a polynucleic acid usually expressed in mole fraction (or percentage) of total bases; the melting temperature of such biopolymers varies with the GC content.

GC content

The percentage of nitrogenous bases on double-stranded DNA which is either guanine and cytosine, a datum of interest to molecular biologists as guanine and cytosine bind to each other with 3 hydrogen bonds (in contrast to adenine and thymine, which share 2 hydrogen bonds), and thus is a benchmark of molecular stability.
References in periodicals archive ?
The GC content of the work is: - creation of 2 bubbling vats 35 m3 - compliance of HTA galleries 30m - creation of a CCD hall 45 mA - separation of common modes - compliance of low-floor rooms of HTA - creating a local TSA - noise processing - processing of breakdowns - Compliance of TR tray - Creation of 2 HTA grids - general management.
Data from comparisons of these kits with similar kits on the market clearly indicates that Norgen's kits yield significantly more diverse cfcRNA, owing to the fact that Norgen's kits use a proprietary resin technology that has the ability to bind all RNA sequences irrespective of size or GC content.
Because our experimental setup introduced only small differences in primer GC content and amplicon length, we observed no effects of these features on any of the studied parameters (see Supplemental Results in the online Data Supplement).
The lowest GC content was in regions A and C, indicative of genes in these regions resulting from horizontal recombination (Table 2; Table 3, Appendix, wwwnc.
As a gene synthesis expert, GenScript presented technical breakthroughs in synthesizing long (>10 kb), complex genes (having high GC content, highly repetitive sequences, complex secondary structures or unstable structural elements), one of the most challenging tasks in synthetic biology research.
These bands pattern could be caused of nucleotide sequences and GC content of each of them, as high GC content of primer AP3 (80%) caused the most bands in compare to low GC content of API and AP2 (60%), produces lower DNA fragments.
High GC containing profiles tend to shift toward higher GC content, an indication of poor base representation.
It has been postulated that the first intron of a gene is usually different from rest of the introns both in size and GC content.
A frequent characteristic of spacers is a balanced GC content between ITS1 and ITS2, and this also occurs in the Veneridae spacers, this fact could indicate the coevolution between the two spacers at the level of base composition.
The GC content of functional siRNA: The optimal GC content of siRNA should be between 30% and 55% [10,14,15].
Customers can verify the GC content, Molecular Weight, and TM of their oligo sequences.
Accuracy and reproducibility are essential to NGS library quantitation methods - the NEBNext Library Quant Kit delivers both, for libraries with a broad range of insert sizes and GC content.