Physical characteristics of the primers, such as melting temperature (Tm), primer length, GC content
(%), molecular weight (g/mole), and the extinction coefficient (L/mole*cm, nmole/ and ug/ ) were presented in Table 5.
Regions that are difficult to interrogate by NGS, such as those with high or low GC content
and homologous regions, are particularly important to assess during the assay validation, especially in a methods-based validation approach.
The GC content
of the host-derived sequences, in most cases, existed in about 40%, while GC content
of the symbiont was in about 54% (Fig.
A common challenge in diagnostics is generating sufficient reads through the first exons of genes, where GC content
tends to be higher than average.
The genomic GC content
was about 40~45% in vertebrate.
HRMA measures the decrease of fluorescence as double-stranded DNA denatures into single-stranded DNA, producing sequencespecific melting profiles that are dependent on amplicon lengths and GC content
Amplicons were designed to include variable characters, lengths, and GC content
The GC content
of the work is: - creation of 2 bubbling vats 35 m3 - compliance of HTA galleries 30m - creation of a CCD hall 45 mA - separation of common modes - compliance of low-floor rooms of HTA - creating a local TSA - noise processing - processing of breakdowns - Compliance of TR tray - Creation of 2 HTA grids - general management.
The result of MIRA assembly found the 242 contigs while largest contig was of 96,232bp with N50 and N90 value of 32,253bp and 9,960bp and GC content
of up to 55% respectively.
BGI's technology involves use of a core algorithm for determining GC content
differences in chromosomes, improving accuracy of the result.
The lowest GC content
was in regions A and C, indicative of genes in these regions resulting from horizontal recombination (Table 2; Table 3, Appendix, wwwnc.
Computational analyses predicted a putative promoter region in location of -561 to +101 relative to the TSS of PeP gene (Figure 1A) with high GC content