GC content

GC con·tent

the amount of guanine and cytosine in a polynucleic acid usually expressed in mole fraction (or percentage) of total bases; the melting temperature of such biopolymers varies with the GC content.

GC content

The percentage of nitrogenous bases on double-stranded DNA which is either guanine and cytosine, a datum of interest to molecular biologists as guanine and cytosine bind to each other with 3 hydrogen bonds (in contrast to adenine and thymine, which share 2 hydrogen bonds), and thus is a benchmark of molecular stability.
References in periodicals archive ?
Physical characteristics of the primers, such as melting temperature (Tm), primer length, GC content (%), molecular weight (g/mole), and the extinction coefficient (L/mole*cm, nmole/ and ug/ ) were presented in Table 5.
Regions that are difficult to interrogate by NGS, such as those with high or low GC content and homologous regions, are particularly important to assess during the assay validation, especially in a methods-based validation approach.
A common challenge in diagnostics is generating sufficient reads through the first exons of genes, where GC content tends to be higher than average.
The GC content of the host-derived sequences, in most cases, existed in about 40%, while GC content of the symbiont was in about 54% (Fig.
The genomic GC content was about 40~45% in vertebrate.
HRMA measures the decrease of fluorescence as double-stranded DNA denatures into single-stranded DNA, producing sequencespecific melting profiles that are dependent on amplicon lengths and GC content (18-20).
The GC content of the work is: - creation of 2 bubbling vats 35 m3 - compliance of HTA galleries 30m - creation of a CCD hall 45 mA - separation of common modes - compliance of low-floor rooms of HTA - creating a local TSA - noise processing - processing of breakdowns - Compliance of TR tray - Creation of 2 HTA grids - general management.
The result of MIRA assembly found the 242 contigs while largest contig was of 96,232bp with N50 and N90 value of 32,253bp and 9,960bp and GC content of up to 55% respectively.
BGI's technology involves use of a core algorithm for determining GC content differences in chromosomes, improving accuracy of the result.
The lowest GC content was in regions A and C, indicative of genes in these regions resulting from horizontal recombination (Table 2; Table 3, Appendix, wwwnc.
Computational analyses predicted a putative promoter region in location of -561 to +101 relative to the TSS of PeP gene (Figure 1A) with high GC content (approximately 71.