GATA5

GATA5

A gene on chromosome 20q13.33 that encodes a member of the GATA family of zinc finger transcription factors, which binds to hepatocyte nuclear factor-1alpha (HNF-1alpha), an interaction required to activate the intestinal lactase-phlorizin hydrolase promoter. GATA5 also binds to the CEF-1 nuclear protein-binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer, and may be involved in pathways that result in smooth muscle diversification.
References in periodicals archive ?
Cardiac Tbox factor Tbx20 directly interacts with Nkx2-5, GATA4, and GATA5 in regulation of gene expression in the developing heart," Developmental Biology, vol.
a (30,31) (a possible ortholog of vertebrate GATA4, GATA5, and GATA6; the original name was Gata-a), and the Zic-like protein Macho-1/Zic-r.
The aberrant DNA methylation of certain promoter regions is linked to tumor formation, owing to the silencing of the transcription of well-established tumor suppressor genes, such as CDKN2A [4] [cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)], GATA5 (GATA binding protein 5), and DAPK1 (death-associated protein kinase 1) (9-11).
We designed 3 assays to detect hypermethylation in the promoters of 3 genes recognized to be involved in lung carcinogenesis (DAPK1, GATA5, and CDKN2A).
We used 1 or 2 [micro]L (approximately 25-50 ng) of DNA as the target for investigating the hypermethylation status of the CDKN2A, DAPK1, and GATA5 promoters.
MS-LAMP primers (F3, B3, FIP, BIP, LF, LB), which specifically amplify only the methylated sequences of the CDKN2A, DAPK1, and GATA5 promoters after bisulfite treatment, were designed with the aid of PrimerExplorer V4 (Eiken).
The sequence of the fluorescent primer used in the GATA5 assaywas shifted 2 nucleotides upstream and lengthened by 1 nucleotide at the 3' end compared with the original LF primer (new sequence, 5' -CAAACCCCGCGAACAAAA-3') to ensure a closer proximity to guanine nucleotides; this primer was labeled at the 5' end with BODIPY 650/665.
The Gata5 gene expresses the protein GATA5, which is a member of a family of zinc-finger transcription factors-proteins that act as switches to turn gene activity on or off.
We first validated this new assay on DNA with known methylation status and then demonstrated the detection of methylation for 3 tumor suppressor genes that are often found to be methylated in lung adenocarcinoma: CDKN2A, RASSF1, and GATA5 (GATA binding protein 5).
We performed MS-FLAG assays on 1 [micro]L bisulfite-treated DNA (corresponding to approximately 5 ng starting material) to investigate hypermethylation of promoter regions of RASSF1, p16, and GATA5.
We used fluorescein for p16 and RASSF1 assays and MAX (Integrated DNA Technologies) dye for GATA5 assays.
The reaction mixture in this case contained 300 nmol/L GATA5 primers (CB131QR and CB132QR) labeled with MAX dye, 200 nmol/L RASSF1 primers (CB107QF, CB108QF) labeled with fluorescein, 500 /,mol/L dNTPs, and 1x JumpStart PCR buffer with 10 units PspGI enzyme (New England Biolab) and 1 unit JumpStart Taq Polymerase (Sigma) in a final volume of 20 [micro]L.