When GATA3 is present, it turns off many genes that are active in metastasis.
The key finding of the new study is that GATA3 acts downstream biochemically to activate a molecule - obscure until now - called microRNA29b.
GATA-2 (Forward: 5'-TGC AAC ACA CCA CCC GAT ACC-3'; Reverse: 5'-CAA TTT GCA CAA CAG GTG CCC-3'), GATA3
(Forward: 5- TCT CAC TCT CGA GGC AGC ATG A-3'; Reverse: 5- GGT ACC ATC TCG CCG CCA CAG-3').
Th1 cells express T-bet and secrete IFN-[gamma]; Th2 cells express GATA3
and secrete IL-4 (7).
Across the four subtypes, mutations in only three genes - TP53, PIK3CA and GATA3
- occurred in more than 10 percent of patients' tumors.
The variations in the expression of 17 genes (underlined in Table 2) are involved in specific neuronal pathways, such as neuronal cell structure (GPM6B, PRPH, RELN), neural signaling and development (HOXB2, ELAVLI, EFNB1, NRG2, NELL1, MDK, GFRA1), synaptogenesis and steroid biosynthesis (SYCP2, PCDH17, STX1B2, SCG5, Nstage 4 diseasel, GATA3
It organizes caudal descent of the nephric duct with PAX8 and GATA3, emergence of the ureteric bud, and induces WT1 in metanephric blastema.
Selected Examples of Genes in Which Mutations can Result in Various Congenital Abnormalities of the Kidney and Urinary Tract (CAKUT) Lesions CAKUT PAX2 TCF2 EYA1 SIX1 SALL1 Dysplasia X XX XX Agenesis X X Hypoplasia X X X UPJ obst X X X VU reflux X GCKD X Syndrome Renal-coloboma MODY5 BOR BOR Townes-Brock CAKUT GATA3 Dysplasia X Agenesis X Hypoplasia UPJ obst VU reflux X GCKD Syndrome HDR Abbreviations: BOR, branchial-oto-renal syndrome; GCKD, glomerulocystic kidney disease; HDR, hypoparathyroidism, deafness and renal dyspla- sia; MODY, maturity onset diabetes type 5; UPJ obst, ureteropelvic junction obstruction; VU, vesicoureteral.
A critical role for NF-kappa B in GATA3
expression and TH2 differentiation in allergic airway inflammation.
Mutations in another gene, GATA3
, also appeared to predict a good response to aromatase inhibitor therapy, while those in MALAT1, a long stretch of non-coding RNA, seemed to be associated with poor outcomes.
The researchers showed in isolated human cells that interferon blocks the development of nascent Th2 cells and inhibits cells that already have become Th2 cells by interfering with a regulatory protein called GATA3
, a transcription factor Th2 cells express to regulate their function.
Researchers at Mayo Clinic's campus in Florida describe a gene called GATA3
that has been silenced in clear cell renal cell carcinoma (ccRCC), the most common kind of kidney cancer, and is a key gene also lost in breast cancer.