GAPDH


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GAPDH

A gene on chromosome 12p13 that encodes glyceraldehyde-3-phosphate dehydrogenase, which has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. It catalyses the reversible
oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide, an important energy-yielding step in carbohydrate metabolism. GADPH participates in nuclear events, including transcription, RNA transport, DNA replication and apoptosis.
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GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues.
Oligonucleotide primers used for quantitative RT-PCR RNA target Primer sequences TNFSF15 Forward 5'-CCTGAGTATTCCAGCAACGCA-3' Reverse 5'-ATCCACCAGCTTGATGTCACTAAC-3' IL-8 Forward 5'-GGCTTGCTAGGGGAAATGA-3' Reverse 5'-AGCTGACTCTGACTAGGAAACTGT-3' GAPDH Forward 5'-GGTGGTGCTAAGCGTGTTAT-3' Reverse 5'-ACCTCTGTCATCTCTCCACA-3' RNA target PCR product size (base pair) GenBank accession no.
The thermal profile for GAPDH was performed for 28 cycles with annealing at 58 [degrees]C for 30 s and extension at 72 [degrees]C for 15 s.
In addition, GAPDH and 18S rRNA had been stably expressed in a comparative transcriptomics study of carp gonads in our laboratory (unpublished data), and the primer for 40S was acquired from Pionnier et al.
PCR amplification of chemokines and GAPDH in Macrophages
Abbreviations: FGFR, fibroblast growth factor receptor; FRS2[alpha], FGFR substrate protein-2[alpha]; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; NF-[kappa]B, nuclear factor kappa-light-chain-enhancer of activated B cells; P-gp, P-glycoprotein; MDR, multidrug resistance; ChIP, Chromatin immunoprecipitation.
Rasband, Research Services Branch, NIMH, National Institutes of Health, Bethesda, MD) and normalized to the signal intensity of GAPDH for equal protein loading control of each sample in each experiment.
Following reverse transcription, the cDNA was used as template for amplification of CC chemokine ligand 3 (CCL3) and GAPDH sequences using the SYBR FAST quantitative polymerase chain reaction (PCR) master mix (KAPA Biosystems, MA, USA) following the manufacturer's protocol.
b) We evaluated RT variability by carrying out RT-qPCR assays targeting GAPDH (glyceraldehyde-3-phosphate dehydrogenase) [5] #1 on 10 cDNA replicates from of each of 6 RTases.
Housekeeping genes, such as [alpha]-TUB ([alpha]-Tubulin), [beta]-actin (Beta actin), 18s rRNA (18s ribosomal RNA), 28s rRNA (28s ribosomal RNA), GAPDH (Glyceraldehyde-3-phosphatedehydrogenase), G6PDH (Glucose-6-phosphate dehydrogenase), [beta]-TUB (Beta-Tubulin), RPL32 (Ribosomal protein L32), EF1-[alpha] (Alpha elongation factor), ELF1-[beta] (beta elongation factor), are usually used as endogenous controls for normalization of qRT-PCR data, but the reference gene expression levels can differ under different environment conditions (Radonic' et al.
Finally, the new multiplex solutions include off-the-shelf TaqMan RNaseP and GAPDH QSY Assays, which quantify GAPDH and RNAseP genes, and are also available in a primer-limited format.
0 (Applied Biosystems) was applied on the sequence of the PEBNA-1 to obtain MGB (Minor Groove Binding) type primers/probe, for the EBNA-1-reaction and on a conserved portion of the reference sequence of human GAPDH (AC 000144, GenBank) to obtain TAMRA (tetramethylrhodamine) type primers/probe for the GAPDH-reaction.