LGALS4

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LGALS4

A gene on chromosome 19q13.2 that encodes a galectin family-type carbohydrate-binding protein, which modulates cell-cell and cell-matrix interactions. LGALS is only expressed in the (large and small) intestine, and is under-expressed in colorectal cancer.
References in periodicals archive ?
Goat Rheb or FKBP38 in the GAL4 activation domain of pGADT7, pretransformed into the yeast strain Y187, were screened by yeast two-hybrid assay by mating them to GAL4BD-FKBP38/Rheb-expressing Y2HGold on SD/Leu-Trp (DDO) plate per the manufacturer's instructions.
Females homozygous for mRb were crossed with males homozygous for the transcription factor GAL4.
Both are a combination of GAL4 DNA binding domain (GAL4-DBD) fusion protein; the N-terminal region of ER[alpha] possessed an autonomous and ligand-independent activity in HeLa cells, but not ERP.
The human ligand-binding domain of each nuclear receptor was expressed as a chimera with the yeast GAL4 DNA-binding domain that activated in trans a 5XUAS-TATA promoter, which regulated the transcription of a reporter sequence unique to each NR RTU.
Each of these lines stably express the ligand-binding domain (LBD) of the specific human nuclear receptor fused to the DNA-binding domain (DBD) of GAL4 and a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS).
We tested effects of DDT on CBP activity using a mammalian one-hybrid assay in which the full-length CBP is tethered to GAL4-DBD in conjunction with a GAL4 responsive luciferase reporter.
Activation of the C-terminal of CBP by DDT was tested using a deletion mutant of CBP containing amino acids 1300-2441 in a GAL4 fusion vector (Figure 5A).
hPXR and mPXR expression vectors; GAL4 DNA-binding domain (DBD)- linked nuclear receptor ligand-binding domain (LBD) vectors; VPl6-hPXR, GAL4-NCoR, GAL4-SMRT, GAL4-SRC1, GAL4-PBP, and CMX-[3-galactosidase expression vectors; and hPXR reporter (CYP3A4XREM-luciferase), mPXR reporter [(CYP3A2)3-luciferase], and GAL4 reporter (MH 100-lucif erase) have been described previously (Blumberg et al.
Martin demonstrated this by using COS cells transfected with chimeric receptors that contain an estrogen-binding domain linked to a GAL4 DNA binding domain.
Using the yeast-two hybrid assay (GAL4 DBD-ER LBD and GAL4 AD-coactivator fusion proteins), Nishikawa et al.