LGALS1

(redirected from GAL1)

LGALS1

A gene on chromosome 22q13.1 that encodes a galectin family-type carbohydrate-binding protein, which is implicated in modulating cell-cell and cell-matrix interactions, and may regulate apoptosis, cell proliferation and cell differentiation.
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The reading frames from these two cDNA were inserted into the pYES2 vector under control of the GAL1 gene and expressed in the appropriate strain of S.
Galactose was added to a final concentration of 2% (wt/v) to induce the yeast GAL1 promoter.
To do this, we amplified the KIN3 gene from strain Y2454, cloned the amplification product into a Topo-TA vector, and then directionally cloned into the pYES vector, putting KIN3 under control of the GAL1 promoter.