A dose of Fungizone
was administered instead of AmBisome in six cases, leading to the two deaths.
BSC-40 cells were grown in Eagle's minimum essential medium (Invitrogen) supplemented with 5% fetal bovine serum (Cultilab, Sao Paulo, Brazil); 25 mg/mL Fungizone
(amphotericin B) (Cristalia, Sao Paulo, Brazil); 500 U/mL penicillin; and 50 mg/mL gentamicin (ScheringPlough, Sao Paulo, Brazil).
Acute nephrtotoxic dose of 4mg/kg body weight was diluted in 5% Dextrose water in accordance with the instructions given by the manufactrur of Fungizone
Dengue virus detection through reverse-transcription PCR (RT-PCR): Mosquito pools were homogenized with a pestle in Leibowitz medium (L-15) supplemented with Fetal Bovine Serum 2% (FBS), Penicillin-Streptomycin (1%) and Fungizone
25 mg/ml fungizone
, 25 mg/ml gentamicin; all from BioWhittaker, USA) at 37[degrees]C in a 5 per cent C[O.
Confusion arose over the drug as the original "conventional" Amphotericin, branded Fungizone
, is prescribed at around one milligram per kg of a patient's weight.
Growth medium (complete DMEM which was supplemented with 100 U/ml penicillum G, 100 [micro]ml streptomycin, 5 [micro]/mo fungizone
, 15 mM HEPES and 10% fetal bovine serum) was then added.
For controls, we added 50 [micro]L of Eagle's minimum essential medium enriched with 5% fetal bovine serum, 1% penicillin-streptomycin, 1% L-glutamine 200 mmol/L, 1% kanamycin, and 3% fungizone
100 for reporting of cases, use of Miltefosin and Fungizone
for treatment, etc.
The original "conventional" Amphotericin, branded as Fungizone
, is prescribed at around one milligram per kg of a patient'sweight,whilemodern forms are branded asAmbizone and Ambleset, with dosages of three milligrams per kg and five milligrams per kg.
, a brand name of amphotericin B dehydroxycholate, manufactured by Sarabhai Chemicals, was widely used in the treatment of kala-azar and PKDL.
2] incubator, in DMEM-Glutamax-I medium supplemented with 100 mL/L inactivated fetal calf serum, fungizone