Fluorescein is included in Table 1 as a reference fluorophore
Autofluorescence spectra excited at 405, 473, and 559 nm covered the entire emission spectra of DAPI, FITC, and Texas Red respectively, indicating broader AF emissions than from these 3 fluorophores
Let J(x,y,z) and K(x,j,z) denote the radiant intensity and the mass density of the fluorophores
, respectively, depending on the spatial coordinates x, y and z.
are particularly sensitive to their local environment, FDOT holds the potential to provide information about tumor physiology, including tissue oxygen, tissue pH and tissue calcium concentration levels.
By labeling these all with the same fluorophore
and making them adjacent and of similar hybridization characteristics, we've now amplified our fluorescent signal in a small physical region enough to make it visible.
Combining both spectral data sets would increase available spectral information unique to different labels, and consequently increase spectral discrimination of several fluorophores
present in a single study.
Initially, the formation of the hairpin approximates the fluorophore
and the quencher molecule in close vicinity, thereby enabling an efficient FRET-based quenching of the fluorophore
When you excite a fluorophore
with a laser it will emit a bunch of photons with a fairly broad distribution of wavelengths.
A procedure is described for assigning the number of equivalent reference fluorophores
(ERF) values to microspheres labeled with a fluorophore
designed to produce a fluorescence response in a given fluorescence channel of a multicolor flow cytometer.
In the usual case of fluorescence emission, a fluorophore
that absorbs a single photon of light at a certain wavelength moves to a higher energy state.
The first used the fluorophore
PGI to measure production of the ROS hydrogen peroxide.
The collisional quenching is a function of the fluorophore
used, its fluorescence lifetime, and the quencher diffusion coefficients.