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luciferase
(redirected from Firefly luciferase)

   Also found in: Dictionary/thesaurus, Encyclopedia, Wikipedia 0.03 sec.
luciferase
(loosif´rās´),
n an enzyme present in certain luminous organisms that act to bring about the oxidation of luciferins; energy produced in the process is liberated as bioluminescence.


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We transiently transfected ER-negative HeLa cells with an expression vector for human ER[alpha] or ER[beta] along with an estrogen-inducible 3X-ERE-TATA-Luc firefly luciferase reporter plasmid and a constitutively active cytomegalovirus (CMV)-[beta]-galactosidase reporter plasmid and treated the cells for 24 hr with a solvent control or increasing concentrations of [E.
The resulting transfectants expressed generally increased, but variable levels of firefly luciferase from the construct containing the GA sequence, at a pH of 6.
 
 
 
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