Subsequently, SH-SY5Y cells were transiently transfected with a dual reporter-plasmid encoding for both, a firefly luciferase
under the control of the promoter of human ADAM10 and a Renilla luciferase under the control of the promoter of human BACE-1 (Reinhardt et al.
coli strain BL21 with pET16b plasmid containing firefly luciferase
was grown in LB medium and purified by Ni-Sepharose affinity chromatography, as reported earlier .
donovani cell lines expressing firefly luciferase
reporter gene (luc.
The relative light units were calculated by normalizing the firefly luciferase
activity against the [beta]-galactosidase activity to evaluate the transfection activity.
An enzymatic cycling method using pyruvate orthophosphate dikinase and firefly luciferase
for the simultaneous determination of ATP and AMP (RNA).
Our in vitro cell-culture-based assay used a firefly luciferase
reporter gene driven by an androgen-responsive promoter.
The assay is ideal for screening in applications where a smaller molecular size reporter gene, compared to the typical firefly luciferase
, is desirable.
PCR products were digested with these restriction enzymes and then cloned into the multiple cloning sites of the pGL3-basic reporter plasmid (promega, USA) to create the MDR1-1855, MRP1-1999, and BCRP-1989 firefly luciferase
In the patented XDS CALUX(R) process, firefly luciferase
is produced when dioxin- like chemicals are presented to the cells.
The luciferase reporter vectors pGL3-Basic (containing the firefly luciferase
gene) and pRL-SV40 (containing the Renilla luciferase gene, transfections and toxicity control), and the dual-luciferase reporter assay system were purchased from Promega (Madison, WI, USA).
Continuous monitoring of ATP converting reactions by purified firefly luciferase
In this study, SUM149 cells were stably transfected with Red Fluorescent Protein (RFP) and Renilla luciferase to establish dual reporter line termed RR, or infected with lentivirus encoding Green Fluorescent Protein (GFP) and Firefly luciferase
to establish a line derivative termed GL.