Feulgen reaction


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Feul·gen stain

(foyl'gen),
a selective cytochemical reaction for DNA in which sections or cells are first hydrolyzed with hydrochloric acid to produce apurinic acid and then are stained with Schiff reagent to produce magenta-stained nuclei; generally the concentration of DNA in nucleoli and mitochondria is too low to permit detection by this stain
See also: Kasten fluorescent Feulgen stain.

Feulgen reaction

An aniline DNA-specific staining reaction. NUCLEIC ACID turns a reddish-purple colour when in contact with the Feulgen reagent which contains fuchsin and sulphuric acid. (Robert Feulgen, 1884–1955, German physiologist and chemist).

Feulgen,

Robert, German nucleic acid biochemist and cytochemist, 1884-1955, first to detect DNA in cells by a specific cytochemical test.
Feulgen reaction - DNA staining reaction.
Feulgen test
Kasten fluorescent Feuglen stain - see under Kasten
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References in periodicals archive ?
Feulgen reaction specifically stained nuclear chromatin; the aldehyde liberated during hydrolysis is from the deoxypentose but not from ribose.
The most widely used procedure for staining epithelial cell preparations for MN analysis involves a Feulgen reaction to identify the DNA of the nucleus and micronucleus, followed by a counterstain with Fast Green to delineate cell cytoplasm.
The gill tissue was fixed in Carnoy's for 24 h (ethanol-glacial acetic acid, 3:1 v/v) and then stained with the Feulgen reaction (Navarrete et al.
On days two and three, respectively four and two root tips were excised from each bulb and microscopy slides were prepared in accordance with the Feulgen reaction procedure: fixation of the root tips in Carnoy reagent (ethanol 95% and acetic acid 45% , 3:1) for 20 min at room temperature, hydrolysis with 1 N HCl at 60[degrees]C for 7 min.
The blood was dispersed on slides, air dried, fixed in methanol-acetic acid (3:1 v/v) at 4 [degrees]C for 24 h and stained with the Feulgen reaction (hydrolysis with 5N HCl for 60 min at room temperature, staining with Schiff's reagent for 60 min, followed for three washes of 5 min each in sulphurous water).
Sections of spinal cords fixed in 10% neutral formalin and submitted to the Feulgen reaction (according to ref.