Feulgen reaction


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Feul·gen stain

(foyl'gen),
a selective cytochemical reaction for DNA in which sections or cells are first hydrolyzed with hydrochloric acid to produce apurinic acid and then are stained with Schiff reagent to produce magenta-stained nuclei; generally the concentration of DNA in nucleoli and mitochondria is too low to permit detection by this stain
See also: Kasten fluorescent Feulgen stain.

Feulgen reaction

An aniline DNA-specific staining reaction. NUCLEIC ACID turns a reddish-purple colour when in contact with the Feulgen reagent which contains fuchsin and sulphuric acid. (Robert Feulgen, 1884–1955, German physiologist and chemist).

Feulgen,

Robert, German nucleic acid biochemist and cytochemist, 1884-1955, first to detect DNA in cells by a specific cytochemical test.
Feulgen reaction - DNA staining reaction.
Feulgen test
Kasten fluorescent Feuglen stain - see under Kasten
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References in periodicals archive ?
The gill tissue was fixed in Carnoy's for 24 h (ethanol-glacial acetic acid, 3:1 v/v) and then stained with the Feulgen reaction (Navarrete et al.
On days two and three, respectively four and two root tips were excised from each bulb and microscopy slides were prepared in accordance with the Feulgen reaction procedure: fixation of the root tips in Carnoy reagent (ethanol 95% and acetic acid 45% , 3:1) for 20 min at room temperature, hydrolysis with 1 N HCl at 60[degrees]C for 7 min.
Replicate slides with or without the 5% hot TCA extraction were stained to confirm DNA extraction, using the Feulgen reaction for DNA as described elsewhere (Rasch 2003).