Amplification of the 490 bp fragment encompassing ApaI (rs 11168271) and TaqI (rs731236) polymorphic site was performed in 25 [micro]l 1xPCR buffer (MBI, Fermentas
, Lithuania) containing 20 pmol of each primer, 2.
Jack Wheeler, president and chief executive officer of Biopool International/Xtrana, stated: "XTRANA is very pleased to have Fermentas
as a supplier of our unique nucleic acid extraction system.
Web sites such as Computer Town, Red Light Communications, MBI Fermentas
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Southhampton, UK) and SmaI (MBI Fermentas
Vilinius, Lithuania) were used according to the manufacturer's recommendations.
After the PCR,15 [micro]L of PCR product was digested with GsuI (MBI Fermentas
, Hamilton, ON) or MseI (New England Biolabs, Burlington, ON) as directed for 2 h at 37 [degrees]C.
Esant didelei substrato--acetono garu --koncentracijai, fermentas
buvo saturuojamas, t.
Tenders are invited for Purchase of FastDigestA BfaI (FspBI) 50 rxn 1 Fermentas
, FastDigestA BsaAI (Ppu21I) 50 rxn 1 Fermentas
, FastDigestA MnlI 50 rxn 1 Fermentas
, FastDigestA TaqI 400 rxn 1 Fermentas
, dNTP Set 100 mM Solutions 4Eu0.
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5 mM magnesium chloride (MgCl2), 200 [micro]M of each deoxyribonucleotide triphosphates (dNTP), 20 pmol/L of each set of specific primers (metabion international AG, Martinsried, Germany), 1 unit Taq DNA polymerase (MBI Fermentas
, Amherst, NY, USA), and 0.
5 U of Taq DNA polymerase (MBI Fermentas
l Expression Diagnostics Fermentas
Fisher Scientific Food and Drug Administration (US FDA) Fujirebio Furuno Electric Co.
Reverse Transcription: Before RT, the extracted RNA samples were treated by RNase-free DNase I (EN0521; Fermentas
, Opelstrasse 9, Germany) to ensure that the extracted RNA used for the synthesis of cDNA are free of DNA contamination.