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Purification of 24 kDa protein using FPLC: In FPLC method using cationic column, proteins leave the column depending on their ionic strength (Fig.
6/150 column and separated by FPLC with an AKTA Purifier (GE Healthcare, Uppsala, Sweden).
As a result, in the Chamber's view, the DRC cannot be considered to be acting in relation to the specific case before the Court (which is limited to Mr Thomas Lubanga Dyilo's alleged responsibility for the UPC/FPLC'S alleged policy/practice of enlisting into the FPLC, conscripting into the FLPC and using to participate actively in the hostilities children under the age of fifteen between July 2002 and December 2003).
It will provide UNH students and faculty a chance to work with FPLC.
In this work, we have successfully cultured the Phanerochaete chrysosporium (ATCC 20696) and purified two kinds of lignin peroxidase by using FPLC.
Cromatografia por filtracion en gel: se realizo en un sistema FPLC (Pharmacia LKB) utilizando una columna preempacada de 10 x 300 mm de Superosa 6HR (Pharmacia).
Protein recovered from glutathione-Sepharose was further purified by FPLC on Superdex 75 (Amersham Pharmacia Biotech).
The versatile AKTA[TM ]design range of chromatography systems includes AKTAexplorer, ideal for rapid methods development, and AKTA FPLC, the industry standard for laboratory-scale separations.
The procedure was applied to a collection of thirty chromatograms, corresponding to FPLC analyses performed in duplicate on five different protein extracts for each cultivar Amac, Alaska-Sweet, and Midfreezer.
The folded inclusion bodies were purified by FPLC based resource Q column equilibrated with 5 column volumes of dialysis buffer.
2+] ion metallic affinity chromatography (IMAC) gel packed chromatography column (AKTA FPLC, GE-Amersham Healthcare), equilibrated with 50 mM Tris-HCl buffer, pH 8.
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