fluorescence polarization immunoassay

(redirected from FPIA)
Also found in: Dictionary, Thesaurus, Acronyms, Encyclopedia.
Related to FPIA: fluorescence polarization immunoassay, EFPIA

fluorescence polarization immunoassay

, fluorescent polarization immunoassay Abbreviation: FPIA
A means of identifying and quantifying the amount of an antigen in a specimen in which a fluorescently labeled antibody is mixed with a sample thought to contain the antigen is exposed to polarized light. Bound fluorescently labeled antibody reacts to polarized light in a characteristic fashion not demonstrated by unbound antibody, allowing for identification and measurement of the ligand.
See also: immunoassay
Mentioned in ?
References in periodicals archive ?
We evaluated potential interference of Asian, American, and Indian ginsengs on serum digoxin measurement both in vitro and in vivo in a mouse model with the ECLIA-digoxin assay and a turbidimetric assay (TIA) on Bayer's ADVIA Chemistry systems and compared the results with the results obtained by using FPIA.
Because of the small number of laboratories included in each method for each of the surveys, the within-method variation data must be interpreted with caution; however, the FPIA method performed in surveys 2 and 3 consistently had lower CVs (mean, 5.
Our analysis of tHcy values obtained on five pools analyzed in two of three surveys showed that only the FPIA method (CV, 4.
At 33 [micro]mol/L tHcy, values obtained by the FPIA method were significantly lower (P = 0.
Within-method variation was again lowest for the FPIA assay; the mean CV was 4.
To confirm the observation that tHcy values obtained by the FPIA method were lower at increased tHcy concentrations compared with values obtained by HPLC-FD (TBP/SBDF), we reanalyzed each of the 10 pools by both methods over 10 days.
CVs in this same study were only slightly lower with the FPIA method on the Abbott IMx analyzer: within-run CVs were between 1.
06%) with the AxSYM FPIA Amphetamine/Methamphetamine II assay, but gives false-positive results only with high urine concentrations of heptaminol.
We report the observation of false-positive results obtained with the AxSYM (r) FPIA Amphetamine/Methamphetamine II assay (Abbott), which is widely used to detect commonly abused amphetamine compounds in human urine.
Syva Emit 2000 and Roche "on line" subject to less interference by digoxin-like factors than Abbott TDx FPIA in newborns and pregnant women.