As part of the validation, we also conducted the RT-PCR assay with FFPE
tissue samples, frozen tissue samples, and FNAB needle washes.
The NEBNext FFPE
DNA Repair Mix makes it possible to apply today's and tomorrow's next-generation sequencing (NGS) technologies even to compromised FFPE
tissue samples, which we hope will open up new avenues in the analysis of these valuable samples.
The second test to be offered clinically in the United States was the Pathwork Tissue of Origin Test developed by Pathwork Diagnostics for both frozen and FFPE
This study included 20 FFPE
blocks of breast cancer tissue collected at 2 pathology laboratories in Germany: 15 blocks from HELIOS Clinics, Wuppertal (embedded in 2005) and 5 blocks from Charite, Berlin (embedded in 2003).
Amplicons were limited to 60 to 100 by in length because of the degraded condition of the FFPE
Analysis of DNA PCR amplification on paired fresh/ frozen specimens and FFPE
tissue demonstrated higher sensitivity for assays performed on fresh/frozen tissue compared with FFPE
tissue; however, the difference was not statistically significant, possibly because of the low sample size.
The corresponding FFPE
samples were primarily fixed in formalin, embedded in paraffin, and stored at room temperature for the same time period.
3370T - Selective depletion of abundant RNAs to enable transcriptome analysis of low input and highly degraded RNA from FFPE
breast cancer samples.
for Affymetrix, which is particularly for data generated by Affymetrix' genetic analysis platforms in cancer research, comprising CytoScanA Cytogenetics Suite and OncoScanA FFPE
We applied a novel expression assay to simultaneously monitor 502 cancer-related genes in RNAs derived from FFPE
samples, using microarrays assembled on fiber optic bundles.
New Offerings Accelerate the Completion of Studies and Expand FFPE
In Phase I, we developed a manual RNAscope-based assay for light chain mRNAs and demonstrated that it allowed determination of light chain restriction in virtually all types of NHL using FFPE
with 99% concordance with the current gold standard assay flow cytometry using fresh tissue", said Xiao-Jun Ma, Ph.