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FBS |
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| 7 cells, a mouse macrophage cell line (American Type Culture Collection, Rockville, MD, USA), were cultured in DMEM supplemented with 10% FBS. BGM, FRhK, HEK-293, HEL, Hep G2, L20, MA-104, pCMK, and RD-A cell lines were all maintained in modified Eagle medium (MEM) supplemented with 10% FBS. We split the cell suspension into two tubes and diluted in two different media compositions; media 1, as described above, or media 2, which differed only by the FBS manufacturer (BioWhittaker, Walkersville, MD). |
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