A 24-hour-urine adrenal steroid profile by GC-mass spectrometry showed robust Cortisol and cortisone but low precursors and, significantly, excessive 5[alpha]-R activity by increased androsterone relative to etiocholanolone
A random dope test was conducted on the athlete in the last week of June and Inderjeet's ' A' sample tested positive for steroids androsterone and etiocholanolone
In the liver, testosterone is converted by a series of enzymatic steps into androsterone, etiocholanolone
, DHT, and 3-androstanediol.
Simultaneous OF testosterone, epitestosterone, dihydrotestosterone, DHEA, androsterone, etiocholanolone
, and [alpha]- and [beta]-androstanediol quantification collected with the Quantisal device were presented, but never published (40).
and dimethyl sulfoxide were from Sigma Chemical Co.
The research has revealed that in certain rare circumstances, a chemical reaction can occur in the bottle after a urine sample has been provided, whereby two endogenous hormones, androsterone (A) and etiocholanolone
(E), are converted into 19-NA and 19-NE, metabolites of nandrolone that are tested for in doping analysis.
of Median Androgens 24hr Dry DHEA 13% 18% Androsterone 37% 48% Etiocholanolone
57% 69% Testosterone 21% 18% 5a-Ansdrostanediol 55% 60% 5b-Ansdrostanediol 27% 43%
Androgen metabolites are analyzed separately by sex, since male androgen metabolites in this age group are increased from testicular-derived androsterone and etiocholanolone
The testosterone metabolites androsterone and etiocholanolone
can be measured in 24-hour urine, while the best androgen metabolites in the serum to assess are dihydrotestosterone and androstanediol glucuronide, which concentration is on the average double that of testosterone.
13]C values for acetylated androsterone and etiocholanolone
as described previously (13).
Testosterone, testosterone glucuronide (TG), androsterone, androsterone glucuronide (AG), etiocholanolone
glucuronide (EtG), 17[alpha]-hydroxyprogesterone (17[alpha]OHP), and Escherichia coli [beta]-glucuronidase were purchased from Sigma.
Saliva requires careful Etiocholanolone
attention to collection procedures to avoid falsely elevated testosterone levels.